Fosmid cloning technique. High molecular weight, total DNA is subject to pulse-field electrophoresis to recover sheared DNA of 45 to 50 kb. This DNA is used to generate a fosmid library which is selectively screened for cpDNA-containing clones, which are then sequenced, annotated and assembled.
Cattolico et al. BMC Genomics 2008 9:211 doi:10.1186/1471-2164-9-211