Figure 5.

Expression of selected barley WRKY genes (HvWRKY) and their designate orthologs in Arabidopsis (AtWRKY) or rice (OsWRKY). The normalized fold difference (upper diagram) of the signal intensities of each amplicon has been calculated from the RT-PCR experiments (lower diagram) to estimate the expression trajectories of WRKY genes relative to its Actin controls over three different organs in three plant species. RT-PCR analysis (lower panels) with mRNA of barley, Arabidopsis and rice isolated from homologous organs (roots, left; leaves, middle; infructescence, right).