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Open Access Highly Accessed Research article

Phylogenetic and comparative gene expression analysis of barley (Hordeum vulgare) WRKY transcription factor family reveals putatively retained functions between monocots and dicots

Elke Mangelsen1, Joachim Kilian2, Kenneth W Berendzen2, Üner H Kolukisaoglu3, Klaus Harter2, Christer Jansson14 and Dierk Wanke2*

Author Affiliations

1 Department of Plant Biology and Forest Genetics, The Swedish University of Agricultural Sciences (SLU), P.O. Box 7080, SE-750 07 Uppsala, Sweden

2 Center of Plant Molecular Biology (ZMBP), University of Tuebingen, Auf der Morgenstelle 1, D-72076 Tübingen, Germany

3 CELISCA (Center for Life Science Automation), Friedrich-Barnewitz-Strasse 8, D-18119 Rostock, Germany

4 Lawrence Berkeley Laboratory, Earth Science Division, 1 Cyclotron Rd., Berkeley, CA 94720, USA

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BMC Genomics 2008, 9:194  doi:10.1186/1471-2164-9-194

Published: 28 April 2008

Abstract

Background

WRKY proteins belong to the WRKY-GCM1 superfamily of zinc finger transcription factors that have been subject to a large plant-specific diversification. For the cereal crop barley (Hordeum vulgare), three different WRKY proteins have been characterized so far as regulators in sucrose signaling, pathogen defense, and in response to cold and drought. However, their phylogenetic relationship remained unresolved.

Results

In this study, we used available sequence information to identify a minimum number of 45 barley WRKY transcription factor (HvWRKY) genes. According to their structural features, the HvWRKY factors were classified into the previously defined polyphyletic WRKY subgroups 1 to 3. Furthermore, we could assign putative orthologs of the HvWRKY proteins in Arabidopsis and rice. While in most cases clades of orthologous proteins were formed within each group or subgroup, other clades were composed of paralogous proteins for the grasses and Arabidopsis only, which is indicative of specific gene radiation events. To gain insight into their putative functions, we examined expression profiles of WRKY genes from publicly available microarray data resources and found group specific expression patterns. While putative orthologs of the HvWRKY transcription factors have been inferred from phylogenetic sequence analysis, we performed a comparative expression analysis of WRKY genes in Arabidopsis and barley. Indeed, highly correlative expression profiles were found between some of the putative orthologs.

Conclusion

HvWRKY genes have not only undergone radiation in monocot or dicot species, but exhibit evolutionary traits specific to grasses. HvWRKY proteins exhibited not only sequence similarities between orthologs with Arabidopsis, but also relatedness in their expression patterns. This correlative expression is indicative for a putative conserved function of related WRKY proteins in monocot and dicot species.