Table 3

Q-PCR confirmation of microarray data.



Micro-array
 Q-PCR



Gene name
 Gene symbol
 Sequence ID
 Ratio*
 Ratio*
 SEM ±
 p-value†
Mitochondria





Complex I





NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9
 Nduf9b
 XM_216929
 1.47
 1.29
 0.05
 0.001
Complex II





Succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
 Sdhb
 XM_216558
 1.43
 1.32
 0.05
 <0.001
Complex III





Ubiquinol-cytochrome c reductase binding protein
 Uqcrb
 XM_001074024
 1.53
 1.28
 0.05
 0.005
Complex IV





Cytochrome c oxidase subunit VIIb
 Cox7b
 NM_182819
 1.61
 1.36
 0.06
 0.001
Complex V





ATP synthase, H+ transporting, mitochondrial F0 complex, subunit G
 ATP5g
 XM_001075306
 1.52
 1.15
 0.05
 0.11
Metabolism and TCA cycle





Aldo-keto reductase family 1, member B8
 Akr1b8
 NM_173136
 2.13
 2.15
 0.22
 0.006
Malic enzyme 1
 Me1
1.65
 1.76
 0.11
 <0.001
Mitochondrial ribosomes





Mitochondrial ribosomal protein S16
 Mrps16
 XM_001064095
 1.43
 1.20
 0.05
 0.03
Mitochondrial protein transport





Translocase of inner mitochondrial membrane 8 homolog b
 Timm8b
 NM_022541
 1.45
 1.19
 0.06
 0.09
Protein turnover





Proteasome subunit alpha type 3-like
 Psma31
 BN000326
 1.39
 1.24
 0.04
 0.002
Mucosal barrier





Phospholipase A2, group IIA (platelets, synovial fluid)
 Pla2g2a
 NM_031598
 3.73
 4.70
 0.87
 0.03
Trefoil factor 3
 Tff3
 NM_013042
 1.7
 1.21
 0.05
 0.04
Top 10 gene





Retinol binding protein 7
 Rbp7
 XM_575960
 2.06
 3.72
 0.36
 <0.001
Peptide hormone





Proglucagon
 Gcg
 NM_012707
 2.56
 2.91
 0.23
 <0.001

* Ratio FOS diet/Control diet.

± SEM of Q-PCR data is given (n = 12 per group)

† p-value of Q-PCR data is given, the p-value of microarray data were all <0.001.

Rodenburg et al. BMC Genomics 2008 9:144   doi:10.1186/1471-2164-9-144