|
Parameter settings relaxed for round two in the primer design process. |
|
| Product length, round 2 |
80 – 160 nucleotides |
| Primer melting temperature, round 2 |
50.0 – 60.0 ° |
| Max. base repeat, round 2 |
3 (e.g., GGG in a primer is allowed) |
Fredslund BMC Genomics 2008 9:140 doi:10.1186/1471-2164-9-140 |
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