Table 1

Sequence sources and statistics for the three sequential ESTIMA:Songbird assemblies




ESTIMA:Songbird



BUILD 1
BUILD 2
BUILD 3




Sequence Source
Raw reads
After filtering
20 K cDNA microarray
Lund/Affy microarray


SB01
2,880
1,840



SB02
18,720
16,360



SB03 (a)
19,104
17,032



SB03 (b)
9,120
5,717



Duke chromatograms
36,469
23,225



Duke from NCBI
17,312
16,442



SB05
11,328
10,056



SB06
11,040
9,760



Rockefeller
9,845
9,764




Raw reads
40,704
86,293
99,349
# SEQS INTO PTA
35,232
58,457
86,784
Pure singlets
10,123
11,823
13,908
Clustered singlets
1,900
2,457
3,662
Total singlets
12,023
14,280
17,570
Contigs
5,855
8,348
14,088
Total unique sequences
17,878
22,628
31,658
Total redundant sequences
17,354
35,829
55,126
Redundancy
49%
61%
64%

Replogle et al. BMC Genomics 2008 9:131   doi:10.1186/1471-2164-9-131