Open Access Highly Accessed Research article

Salmon louse (Lepeophtheirus salmonis) transcriptomes during post molting maturation and egg production, revealed using EST-sequencing and microarray analysis

Christiane Eichner1, Petter Frost15*, Bjarte Dysvik2, Inge Jonassen24, Bjørn Kristiansen3 and Frank Nilsen16

Author Affiliations

1 Institute of Marine Research, Bergen, Norway

2 Department of Informatics, University of Bergen, Bergen, Norway

3 The Norwegian microarray consortium, Institute for molecular bioscience, University of Oslo and The Norwegian Radium Hospital, Norway

4 Computational Biology Unit, Bergen Center for Computational Science, University of Bergen, Norway

5 Current address: Intervet Norbio AS, Thormohlensgt. 55, N-5008 Bergen, Norway

6 Current address : Department of Biology, University of Bergen, Norway

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BMC Genomics 2008, 9:126  doi:10.1186/1471-2164-9-126

Published: 10 March 2008

Additional files

Additional file 1:

Identification of groups of genes (clusters) with similar expression profiles using a 5 × 5 self-organizing map (SOM). Highest up-regulated profiles are up to the left while max down-regulated can be seen down to the left.

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Additional file 2:

Correspondence analysis (developmental stage and transcription) including all samples. Samples are located in adjacency with other samples from the same developmental group or in the vicinity of a neighboring time point in development.

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Additional file 3:

Self-organizing map (SOM). For each sample, the SOM creates 10 × 10 cells where each cell corresponds to the mean expression value for one neuron in the map. Similar color pattern can be seen for biological replicates (samples within each development group).

Format: PDF Size: 185KB Download file

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Additional file 4:

List of accession numbers and characteristics of all described L.salmonis gene contigs including hyperlinks to GenBank.

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Additional file 5:

Validation of microarray results by comparing microarray transcription profiles with Northern blots for 7 different L.salmonis contigs.

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