Figure 5.

Plot of mean net charge versus mean hydrophobicity of LEA and selected other proteins. "No LEA" refers to proteins originally annotated as LEA proteins but re-annotated in our study (Additional file 1). Arabidopsis seed storage proteins were included in the analysis, because they are a group of seed proteins that have clearly no sequence similarities to LEA proteins. The line marks the border between natively unstructured (left) and folded (right) proteins [91]. The inset documents the shift of five proteins when the putative targeting sequence is removed.