Table 5

TGF-β regulated genes (38) in A549 involved in MAP kinase signalling

Acc No

DAVID Gene Name

1 hr

4 hr

12 hr


H09832

ACTIVIN A RECEPTOR, TYPE IB

1.01 ± 0.17

1.66 ± 0.29

1.17 ± 0.06

N27681

HEAT SHOCK 70 KDA PROTEIN 1A

1.12 ± 0.01

1.46 ± 0.18

1.42 ± 0.08

H73255

MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 6

0.84 ± 0.31

1.04 ± 0.07

1.49 ± 0.11

N42722

GUANINE NUCLEOTIDE BINDING PROTEIN (G PROTEIN), GAMMA 12

1.44 ± 0.04

1.5 ± 0.09

1.28 ± 0.17

AA039598

RIBOSOMAL PROTEIN S6 KINASE, 90 KDA, POLYPEPTIDE 1

1.16 ± 0.13

1.21 ± 0.02

1.32 ± 0.02

AA030029

PROTEIN KINASE C, ALPHA

1.33 ± 0.23

1.56 ± 0.08

1.36 ± 0.03

H73472

CELL DIVISION CYCLE 42 (GTP BINDING PROTEIN, 25 KDA)

1.24 ± 0.27

1.33 ± 0.07

1.56 ± 0.01

H62639

HEAT SHOCK 70 KDA PROTEIN 8

1.29 ± 0.01

1.68 ± 0.18

1.37 ± 0

H23019

TRANSFORMING GROWTH FACTOR, BETA RECEPTOR I (ACTIVIN A RECEPTOR TYPE II-LIKE KINASE, 53 KDA)

1.09 ± 0.01

2.53 ± 0.07

2.11 ± 0.41

AA088648

FIBROBLAST GROWTH FACTOR RECEPTOR 1 (FMS-RELATED TYROSINE KINASE 2, PFEIFFER SYNDROME)

1.45 ± 0.24

1.97 ± 0.08

1.77 ± 0.09

AV747676

V-AKT MURINE THYMOMA VIRAL ONCOGENE HOMOLOG 3 (PROTEIN KINASE B, GAMMA)

1.06 ± 0.13

1.46 ± 0.05

1.18 ± 0.01

AI500475

RAS GUANYL RELEASING PROTEIN 1 (CALCIUM AND DAG-REGULATED)

1.31 ± 0.05

1.37 ± 0

1.41 ± 0.07

R86053

NUCLEAR FACTOR OF KAPPA LIGHT POLYPEPTIDE GENE ENHANCER IN B-CELLS 2 (P49/P100)

0.99 ± 0.12

0.9 ± 0.07

1.44 ± 0.09

BE502112

RIBOSOMAL PROTEIN S6 KINASE, 90 KDA, POLYPEPTIDE 3

0.37 ± 0.1

0.29 ± 0.05

0.41 ± 0.06

H23109

FIBROBLAST GROWTH FACTOR 1 (ACIDIC)

0.94 ±

1.36 ± 0.01

1.13 ± 0.05

AA127934

PROTEIN KINASE, X-LINKED

1.21 ± 0.07

1.38 ± 0.01

1.35 ± 0.07

H89206

PROTEIN KINASE, CAMP-DEPENDENT, CATALYTIC, BETA

1.18 ± 0.08

1.42 ± 0.04

1.25 ± 0.03

R14348

MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5

1.45 ± 0.09

1.22 ± 0.17

1.03 ± 0.06

BM924824

RAS-RELATED C3 BOTULINUM TOXIN SUBSTRATE 1 (RHO FAMILY, SMALL GTP BINDING PROTEIN RAC1)

1.39 ± 0

1.41 ± 0.06

1.28 ± 0.18

H11708

MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4

1.38 ± 0.02

1.54 ± 0.1

1.24 ± 0.17

R36401

FILAMIN B, BETA (ACTIN BINDING PROTEIN 278)

0.96 ± 0.02

1.17 ± 0.11

1.33 ± 0.01

BI758537

MADS BOX TRANSCRIPTION ENHANCER FACTOR 2, POLYPEPTIDE C (MYOCYTE ENHANCER FACTOR 2C)

1.77 ± 0.42

1.25 ±

1.36 ± 0.09

W56249

ACTIVATING TRANSCRIPTION FACTOR 2

1.25 ± 0.13

1.5 ± 0.02

1.72 ± 0.18

AA034981

INTERLEUKIN 1 RECEPTOR, TYPE I

0.37 ± 0.08

0.4 ± 0.02

0.29 ± 0.04

AA056664

V-AKT MURINE THYMOMA VIRAL ONCOGENE HOMOLOG 1

1.34 ± 0.15

1.44 ± 0.05

1.36 ± 0.07

H20677

MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 7 INTERACTING PROTEIN 1

1.13 ± 0.15

0.96 ± 0.1

1.6 ± 0.01

BM999610

V-JUN SARCOMA VIRUS 17 ONCOGENE HOMOLOG (AVIAN)

1.57 ± 0.26

2.13 ± 0.01

1.66 ± 0.18

BE781203

CALCIUM CHANNEL, VOLTAGE-DEPENDENT, ALPHA 1G SUBUNIT

1.13 ± 0.03

1.02 ± 0

1.44 ± 0

T83272

RAS P21 PROTEIN ACTIVATOR 2

0.96 ± 0.08

1.12 ± 0.18

1.33 ± 0.02

T75436

MITOGEN-ACTIVATED PROTEIN KINASE 10

1.16 ± 0.17

1.22 ± 0.05

1.37 ± 0.07

W70006

CELL DIVISION CYCLE 25B

1.47 ± 0.1

1.15 ± 0.05

1.38 ± 0.08

R32409

PHOSPHOLIPASE A2, GROUP V

1.14 ± 0.27

1.27 ± 0.01

1.5 ± 0.07

AW961873

CALCIUM CHANNEL, VOLTAGE-DEPENDENT, GAMMA SUBUNIT 4

1.45 ± 0.15

1.23 ± 0.17

1.02 ± 0

BI752921

PLATELET-DERIVED GROWTH FACTOR RECEPTOR, BETA POLYPEPTIDE

1.43 ± 0.09

1.03 ± 0.02

1.09 ± 0.07

BQ055308

RIBOSOMAL PROTEIN L4

1.44 ± 0.05

1.26 ± 0.04

1.43 ± 0.08

R14058

PROTEIN KINASE C, BETA 1

1.54 ± 0.06

1.08 ± 0.01

1.15 ± 0

BQ016117

V-FOS FBJ MURINE OSTEOSARCOMA VIRAL ONCOGENE HOMOLOG

1.48 ± 0.26

1.44 ± 0.04

1.29 ± 0.06

W05234

CALCIUM CHANNEL, VOLTAGE-DEPENDENT, BETA 3 SUBUNIT

1.4 ± 0.07

1.15 ± 0.05

1.27 ± 0


The list in the table shows the regulated genes as revealed by the DAVID tool. The columns show Genbank accession no., gene name, and fold change with respect to untreated controls at 1 hr, 4 hr and 12 hr time points following TGF-β treatment respectively. The regulation shown against each gene is depicted as fold change ± S.E. with respect to untreated cells. Each experimental value of the duplicates of respective genes is shown in tables S1 and S2 (see additional files 1 and 2). The number in the parenthesis of the title represents the number of genes in the table.

Ranganathan et al. BMC Genomics 2007 8:98   doi:10.1186/1471-2164-8-98

Open Data