Additional file 5.
Induction of pSBE-Luc activity by TGF-β in A549 cells. For SBE-luc induction by TGF-β in A549 cells, twenty five thousand cells were plated in 24 well dishes 16–24 hours prior to transfection. 200 ng of pSBE-luc plasmid and 1.25 ng of pRL-CMV construct (Renilla luciferase, for transfection normalization) were transfected using Effectene reagent (Qiagen GmbH, Germany) in serum free conditions for 12 hrs. The cells were recovered in medium containing 10% FBS for 24 hours, washed with serum free medium for 24 hours (3 changes) and then treated with 5 ng/ml TGF-β for 18 hours. The cells were then lysed and lysates were used for dual-luciferase assay (Promega inc, USA). The ratio of the firefly-luciferase to renilla-luciferase has been plotted on the y-axis.
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Ranganathan et al. BMC Genomics 2007 8:98 doi:10.1186/1471-2164-8-98