qRT-PCR analyses of selected genes (TMEPA, TGFBIP, IGFBP7, Integrin αV, TSP-1, MMP2, TGM2) with respect to TGF-β treatment in HPL1D cells. The cells were grown to 90% confluence, washed with serum free medium and treated with 5ng/ml TGF-β 1 for 1, 4, 6, 12 and 24 hours along with untreated controls at each time-point. Two microgram of total RNA from each treatment was reverse transcribed and cDNA equivalent to 10ng total RNA was used for the PCR reactions. The graphs represent the fold change over untreated controls after normalization with the expression of RPL35a. The bars show mean ± SD of two experiments of the PCR reactions done in duplicates.
Ranganathan et al. BMC Genomics 2007 8:98 doi:10.1186/1471-2164-8-98