|
Cytokine genes examined by microarray and rt-PCR in Mayo Clinic kidney transplant specimens. |
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| Microarray detection |
rt-PCR detection |
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| Gene Name |
Probe Set ID |
BLAST of Consensus Probe Sequence OK? |
%P of Mayo samples (n = 30) |
Min Detection p-value |
Max Detection p-value |
%Detected in Mayo samples (n = 29) |
Min Ct |
Max Ct |
|
|
|
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| 1 |
TNF-α |
207113_s_at |
Yes |
0.0% |
0.0806 |
0.3989 |
100% |
30.03 |
36.48 |
| 2 |
IFNγ |
210354_at |
Yes |
0.0% |
0.0562 |
0.5676 |
100% |
32.97 |
41.78 |
| 3 |
IL-6 |
205207_at |
Yes |
70.0% |
0.0007 |
0.1116 |
100% |
30.83 |
39.89 |
| 4 |
IL-10 |
207433_at |
Yes |
0.0% |
0.1116 |
0.6963 |
100% |
32.82 |
37.16 |
| 5 |
TGF-β 1 |
||||||||
| 203085_s_at |
Yes |
0.0% |
0.1497 |
0.9324 |
100% |
26.55 |
32.54 |
||
| 203084_at |
Yes |
0.0% |
0.1497 |
0.6338 |
100% |
26.55 |
32.54 |
||
| 6 |
FoxP3 |
||||||||
| 224211_at |
Yes |
0.0% |
0.2742 |
0.8286 |
100% |
30.87 |
37.22 |
||
| 221333_at |
NOa |
0.0% |
0.6655 |
0.9539 |
- |
- |
- |
||
| 221334_s_at |
NOa |
0.0% |
0.0806 |
0.6655 |
- |
- |
- |
||
| 7 |
IFNγ-R1 |
||||||||
| 202727_s_at |
Yes |
100.0% |
0.0002 |
0.0020 |
100% |
28.53 |
33.95 |
||
| 242903_at |
NOb |
100.0% |
0.0002 |
0.0376 |
- |
- |
- |
||
| 211676_s_at |
Yes |
96.7% |
0.0007 |
0.0676 |
100% |
28.53 |
33.95 |
||
|
a Contains probesets for the FoxP3 gene which overlap with the JM2 gene. b Contains probesets for the IFNγ-Receptor 1 gene that specifically bind the DNA sequence of the gene. | |||||||||
Park and Stegall BMC Genomics 2007 8:88 doi:10.1186/1471-2164-8-88 |
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