Figure 3.

RT-PCR of proapoptotic bbc3, pmaip1, tnfrsf10b and bok expression in mouse skeletal muscle. (A) Relative expression ratio ± SE for mRNA levels of p53-mediated apoptotic genes in young, old, and old CR (n = 5 for each group). The mRNA levels for these four genes were also measured using RT-PCR in 5–30 month old C57BL/6NHsd mouse gastrocnemius muscle (B-E) (mean ± SE of 5 animals per age group). Two-tailed Student's t-test; *P < 0.05, **P < 0.005 (young verses old), ^†P < 0.05 (old versus old CR).