Open Access Highly Accessed Research article

Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation

Birgit Kulterer1, Gerald Friedl2, Anita Jandrositz3, Fatima Sanchez-Cabo1, Andreas Prokesch1, Christine Paar1, Marcel Scheideler1, Reinhard Windhager2, Karl-Heinz Preisegger3 and Zlatko Trajanoski1*

Author Affiliations

1 Institute for Genomics and Bioinformatics and Christian-Doppler Laboratory for Genomics and Bioinformatics, Graz University of Technology, Graz, Austria

2 Department of Orthopaedics, Medical University of Graz, Graz, Austria

3 Eccocell Biotechnology Inc., Graz, Austria

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BMC Genomics 2007, 8:70  doi:10.1186/1471-2164-8-70

Published: 12 March 2007

Additional files

Additional File 1:

FACS analysis. Surface profiles in different passages.

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Additional File 2:

Expression of stem cell specific marker genes evaluated by Realtime RT-PCR. Expression of stem cell specific marker genes evaluated by Realtime RT-PCR.

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Additional File 3:

Array Design. Information on the spotted oligos.

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Additional File 4:

SOP AMINOALLYL LABELING OF RNA FOR HUMAN OLIGO CHIPS. Standard Operating Procedure for AMINOALLYL LABELING OF RNA FOR HUMAN OLIGO CHIPS.

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Additional File 5:

SOP HUMAN OLIGO CHIP PROBE HYBRIDIZATION. Standard Operating Procedure for microarray hybridization.

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Additional File 6:

Differentially expressed ESTs. Differentially expressed ESTs (>2-foldchange) when comparing p2/p5 and p5/p10 for following moderated t-test

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Additional File 7:

Validation of microarray data by Realtime RT-PCR. Gene expression analysis of long-term cultivated MSC.

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Additional File 8:

Gene expression of osteoblast specific genes determined by Real-time RT-PCR. Gene expression of osteoblast specific genes determined by Real-time RT-PCR to prove the osteogenic phenotype.

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Additional File 9:

Selected ESTs for microarray analysis. 1108 selected ESTs for microarray analysis.

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Additional File 10:

k-means clustering. k-means clustering of 1108 selected ESTs.

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Additional File 11:

Figure of merit analysis. Figure of merit analysis for validation of the k-value.

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Additional File 12:

Hierarchical clustering. Hierarchical clustering of 1108 selected ESTs.

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Additional File 13:

Validation of microarray data using real-time RT-PCR. Gene expression analysis of MSC during osteogenesis.

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Additional File 14:

Functional annotation. Distribution of gene ontology (GO) terms in each cluster.

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Additional File 15:

Expression matrix. Expression matrix of differentially expressed ESTs (>2-foldchange).

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Additional File 16:

Selected ESTs for microarray analysis. 659 selected ESTs for microarray analysis.

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Additional File 17:

Validation of microarray data by real-time RT-PCR. Real-time RT-PCR data.

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Additional File 18:

Evaluation of human MSC as in vitro differentiation model. Comparison of human osteoblasts with in vitro differentiated hMSC.

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