Table 4

Amino acid alignment of three D. pulex metallothionein genes against those from other crustaceans (decapods) and from selected insectsa.

Protein domains

|-------------- Beta --------------| |------------------- Alpha --------------------|


Mtn1 Anopheles gambiae

MP

    C
K
    C
CGN-DCKCTSG---CGSGQPCAT---DCKCA
    C
ASGG
    C
KEKS------------------------------GGCCGK--

Mtn2 Anopheles gambiae

MP

    C
KTCVA-DCKCTSP--NCGAGCGCES---RCTCP
    C
KDGAK----------------------------------EGCCK---

MtnA Drosophila melanogaster

MP

    C
P-CGS-GCKCASQ--ATKGSCNCGS---DCKCG---------------------------------GDKKSA-
    C
GCSE---

MtnB Drosophila melanogaster

MV

    C
KGCGT-NCQCSAQ--KCGDNCACNK---DCQCV
    C
KNGPK----------------------------------DQCCSNK-

MtnC Drosophila melanogaster

MV

    C
KGCGT-NCKCQDT--KCGDNCACNQ---DCKCV
    C
KNGPK----------------------------------DQCCKSK-

MtnD Drosophila melanogaster

MG

    C
KACGT-NCQCSAT--KCGDNCACSQ---QCQCS
    C
KNGPK----------------------------------DKCCSTKN

CuMtn2 Callinectes sapidus CRAB

MP

    C
G-CGT-SCKCGSGKCCCGSTCNCTTCPSKQSCSCNDGA
    C
GSA
    C
--------------Q
    C
KTS
    C
C
    C
GAD
    C
K---
    C
SPCPMK-

Mtn1 Callinectes sapidus CRAB

MPGP

    C
CND-KCVCQEG--GCKAGCQCTS----CRCS-P
    C
QK
    C
TSG
    C
--------------K
    C
ATKEE
    C
SKT
    C
TKP-
    C
SCCPK--

Mtn2 Callinectes sapidus CRAB

MPDP

    C
CND-KCECKEG--ECKTGCKCKS----CRCP-P
    C
DK
    C
SSE
    C
--------------K
    C
TSKEE
    C
SKT
    C
SKP-
    C
SCCP---

Mtn1 Scylla serrata CRAB

-PGP

    C
CND-KCVCKEG--GCKEGCQCTS----CRCS-P
    C
EK
    C
SSG
    C
--------------K
    C
ANKEE
    C
SKT
    C
SKA-
    C
SCCPT--

Mtn2 Scylla serrata CRAB

MPDP

    C
CID-KCDCKEG--ECKTGCKCTS----CRCP-P
    C
EQ
    C
SSG
    C
--------------K
    C
ANKED
    C
RKT
    C
SKP-
    C
SCCP---

Mtn1 Potamon potamios CRAB

-PDP

    C
CAEGTCECEEG--KCKAGCKCTS----CRCS-P
    C
EK
    C
TSE
    C
--------------E
    C
KSKEE
    C
AKN
    C
TKP-
    C
SCCP---

Mtn Carcinus maenas CRAB

MPDP

    C
CID-KCECKEG--GCKAGCKCTS----CRCT-P
    C
EK
    C
SSG
    C
--------------K
    C
TTKED
    C
CKT
    C
TKP-
    C
SCCP---

Mtn Eriocheir sinensis CRAB

MPDP

    C
CND-KCECKEG--KCEAGCKCTS----CRCP-P
    C
EK
    C
SSG
    C
--------------K
    C
GSKED
    C
CKT
    C
SKP-
    C
SCCP---

Mtn Portunus pelagicus CRAB

MPDP

    C
CID-KCECKEG--KCEAGCKCTS----CRCP-P
    C
EK
    C
SSG
    C
--------------K
    C
GSKED
    C
CKT
    C
SKP-
    C
SCCP---

Mtn Panulirus argus LOBSTER

MPGP

    C
CID-KCECAEG--KCKSGCQCKS----CTCSTP
    C
DK
    C
TTA
    C
--------------C
    C
STKEE
    C
ASK
    C
TKP-
    C
KCCP---

Mtn Homarus americanus LOBSTER

MPGP

    C
CKD-KCECAEG--GCKTGCKCTS----CRCA-P
    C
EK
    C
TSG
    C
--------------K
    C
PSKDE
    C
AKT
    C
SKP-
    C
KCCP---

Mtn Astacus astacus CRAYFISH

MPGP

    C
CND-VCECAAG--GCKTGCVCTS----CRCS-P
    C
DK
    C
TSG
    C
--------------K
    C
PSKEE
    C
AKT
    C
SKP-
    C
ECCP---

Mtn1 Daphnia pulex cDNA

MTKD--------------CCQGKCSCGD---NCKCGPN

    C
AQ
    C
PAAA----T
    C
A
    C
ATGGE
    C
K
    C
SGN
    C
Q
    C
STS
    C
PCK-SACCK---

Mtn2 Daphnia pulex cDNA

MPKE

    C
V------------RCQNGCTCGD---DCKCAAN
    C
IK
    C
PTASSQGET
    C
K
    C
STPGGCT
    C
GTN
    C
Q
    C
GAS
    C
V
    C
KASSCCK---

Mtn3 Daphnia pulex cDNA

MPNA

    C
CQN-KCSCGSGCNCCQSKCTCGS---GCKCGPNGAP
    C
QNSA-----
    C
I
    C
ATGGG-G
    C
GSD
    C
R
    C
PTS
    C
G
    C
K-TSCCK---

Mtn3 Daphnia magna cDNA¥

MPNV

    C
CQN-NCSCGNGCTCCQSKCTCGS---GCKCGPNGAP
    C
QNSA-----
    C
I
    C
ATGGG-G
    C
GSD
    C
R
    C
PVS
    C
G
    C
K-TSCCK---

Cysteine residues

  0 00    0 0      0   0 0      0 1 1 1  1   1     1 1       1   1 1   2 2  2 22    

  1 23    4 5      6   7 8      9 0 1 2  3   4     5 6       7   8 9   0 1  2 34    

Metal binding

    **    * *      *   * *      * *   *  *   *               *     *   *    * **    


aCysteine residues are bold for sites that are conserved in crustacean and insect genes (residues 3–10, 23, 24) and

    underlined
when residues are clade-specific. Assuming a correct alignment, residues 2, 12, 13, 17, 19 and 20 are conserved in all (or most) of the crustacean genes, whereas residues 14 and 22 are conserved in the decapod genes. The residues 15, 16, 18 and 21 are exclusively found in the Daphnia genes (representing branchiopods) and based on the limited sampling of insect genes (from two dipterans), residues 1 and 11 are excluded in the crustacean genes. Domain partitions and metal bind residues (*) are based on experimental evidence from decapod proteins (Valls et al. [41]). ¥ Poynton et al. [19] provides full translation.

Shaw et al. BMC Genomics 2007 8:477   doi:10.1186/1471-2164-8-477

Open Data