Table 3

Selection of genes with fold change between unfused and fused sutures and between each unfused suture site.

Gene Name
Affymetrix ID
Description
Fold U-F
P-value U-F
Unfused Lambdoid
Unfused Coronal
Unfused Sagittal

Signalling
     ANGPTNL2
213004_at
angiopoietin-like 2
6.67
0.024
1.00
-1.45
-2.87
     EGFR
201983_s_at
epidermal growth factor receptor
2.71
0.038
1.00
1.02
1.20
     EPHA3
206071_s_at
EPH receptor A3
2.92
0.480
1.00
-2.06
-5.33
     EPHA4
206114_at
EPH receptor A4
2.75
0.173
1.00
-1.19
-1.60
     EPHB2
209589_s_at
EPH receptor B2
2.39
0.093
1.00
1.22
-1.12
     FGFR2
208228_s_at
fibroblast growth factor receptor 2
3.36
0.294
1.00
-1.42
-3.72
     FZD1
204451_at
frizzled homolog 1 (Drosophila)
2.13
0.326
1.00
-1.08
-2.41
     IL11RA
204773_at
interleukin 11 receptor, alpha
5.90
0.003
1.00
1.49
1.32
     TGFB2
209909_s_at
transforming growth factor, beta 2
3.76
0.096
1.00
-1.01
-2.08
     FGFR1
222164_at
fibroblast growth factor receptor 1
1.23
0.480
1.00
-1.29
1.12
     FGFR3
204379_s_at
fibroblast growth factor receptor 3
1.42
0.480
1.00
1.23
1.33
     WNT5A
205990_s_at
wingless-type MMTV integration site family, member 5A
1.24
0.857
1.00
-1.03
-5.18
     IL17R
205707_at
interleukin 17 receptor
-2.67
0.480
1.00
-1.25
3.35
     OLFM4
212768_s_at
olfactomedin 4
-6.69
0.480
1.00
-2.15
6.77
     WIF1
204712_at
WNT inhibitory factor 1
-3.19
0.480
1.00
-1.27
5.37
Structural
     COL2A1
213492_at
collagen, type II, alpha 1 (primary osteoarthritis)
7.21
0.390
1.00
-20.66
-3.84
     COL3A1
215077_at
collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV)
6.32
0.072
1.00
1.24
-3.84
     COL8A2
221900_at
collagen, type VIII, alpha 2
11.49
0.092
1.00
-1.23
-12.73
     COL10A1
217428_s_at
collagen, type X, alpha 1(Schmid metaphyseal chondrodysplasia)
14.65
0.407
1.00
-41.66
-17.28
     FBLN1
202995_s_at
fibulin 1
12.88
0.025
1.00
-1.39
-1.92
     FMOD
202709_at
fibromodulin
5.41
0.024
1.00
-1.09
-1.70
     NELL2
203413_at
NEL-like 2 (chicken)
6.40
0.270
1.00
-4.21
-3.01
     PRELP
204223_at
proline arginine-rich end leucine-rich repeat protein
10.65
0.009
1.00
1.20
-1.31
     THBS2
203083_at
thrombospondin 2
6.62
0.069
1.00
-1.26
-2.47
     COL1A2
202404_s_at
collagen, type I, alpha 2
1.05
0.480
1.00
-1.01
-1.06
     CORO1A
209083_at
coronin, actin binding protein, 1A
-5.08
0.480
1.00
-1.05
11.73
Transcription
     BHLH3
221530_s_at
basic helix-loop-helix domain containing, class B, 3
5.95
0.093
1.00
-2.23
-4.76
     CART1
206837_at
cartilage paired-class homeoprotein 1
2.06
0.533
1.00
16.21
2.38
     HLF
204753_s_at
hepatic leukemia factor
3.54
0.210
1.00
-2.55
-6.81
     JUN
201465_s_at
v-jun sarcoma virus 17 oncogene homolog (avian)
4.41
0.161
1.00
3.14
2.50
     PITX2
207558_s_at
paired-like homeodomain transcription factor 2
3.45
0.279
1.00
1.63
-2.08
     SIX2
206510_at
sine oculis homeobox homolog 2 (Drosophila)
2.72
0.136
1.00
-1.09
-1.70
     FOS
209189_at
v-fos FBJ murine osteosarcoma viral oncogene homolog
1.40
0.745
1.00
14.96
4.13
     FOSB
202768_at
FBJ murine osteosarcoma viral oncogene homolog B
1.83
0.695
1.00
54.37
20.22
     FOXD1
206307_s_at
forkhead box D1
1.49
0.499
1.00
-4.71
-5.17
     JUNB
201473_at
jun B proto-oncogene
1.28
0.703
1.00
3.61
2.95
     MEOX2
206201_s_at
mesenchyme homeo box 2 (growth arrest-specific homeo box)
1.66
0.495
1.00
-3.99
-7.74
     MSX2
210319_x_at
msh homeo box homolog 2 (Drosophila)
1.95
0.464
1.00
-1.14
-2.23
     TWIST1
213943_at
twist homolog 1 (Saethre-Chotzen syndrome)
1.50
0.476
1.00
-1.20
-1.27
     LHX6
219884_at
LIM homeobox 6
-1.80
0.050
1.00
-1.07
-1.03
     SHOX2
210135_s_at
short stature homeobox 2
-3.44
0.480
1.00
-1.00
4.50
     PAX5
221969_at
paired box gene 5 (B-cell lineage specific activator)
-4.20
0.480
1.00
-1.69
6.25
Catalytic Activity
     ROR1
205805_s_at
receptor tyrosine kinase-like orphan receptor 1
2.26
0.095
1.00
1.21
-1.14
     HDHD1A
203974_at
haloacid dehalogenase-like hydrolase domain containing 1A
-1.78
0.018
1.00
1.05
1.05
     ALOX5
204446_s_at
arachidonate 5-lipoxygenase
-5.55
0.480
1.00
-1.30
6.76
     ANXA3
209369_at
annexin A3
-7.17
0.480
1.00
-1.30
6.76
Transporter
     C1QTNF3
220988_s_at
C1q and tumor necrosis factor related protein 3
20.25
0.007
1.00
-1.52
-4.92
     RBP4
219140_s_at
retinol binding protein 4 (plasma) KIAA1922
37.38
0.003
1.00
-1.35
-2.53
     APOC1
204416_x_at
apolipoprotein C-I
-2.01
0.480
1.00
1.08
5.06
Binding
     AGC1
217161_x_at
aggrecan 1 (chondroitin sulfate proteoglycan 1)
6.16
0.024
1.00
-1.25
-1.56
     CCND1
208711_s_at
cyclin D1
2.13
0.449
1.00
1.38
2.24
     GPC3
209220_at
glypican 3
7.13
0.050
1.00
1.26
-3.34
     HAPLN1
205523_at
hyaluronan and proteoglycan link protein 1
7.10
0.050
1.00
-1.96
-4.01
     INHBA
210511_s_at
inhibin, beta A (activin A, activin AB alpha polypeptide)
6.94
0.006
1.00
-1.36
-3.03
     MFAP4
212713_at
microfibrillar-associated protein 4
16.50
0.001
1.00
1.32
-1.86
     OGN
218730_s_at
osteoglycin (osteoinductive factor, mimecan)
5.71
0.166
1.00
-1.41
-4.65
     POSTN
214981_at
periostin (osteoblast specific factor 2)
5.87
0.101
1.00
-1.16
-1.60
     PTN
209466_x_at
pleiotrophin (osteoblast specific factor 1)
6.25
0.031
1.00
-1.14
-1.78
     S100A10
200872_at
S100 calcium binding protein A10 (annexin II ligand)
2.01
0.096
1.00
-1.04
-1.60
     CCND3
201700_at
cyclin D3
-2.60
0.480
1.00
-1.00
3.62
     FCN1
205237_at
ficolin (collagen/fibrinogen domain containing) 1
-5.45
0.480
1.00
-1.46
12.39
     FGR
208438_s_at
Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
-3.03
0.480
1.00
-1.13
5.75
     S100A12
205863_at
S100 calcium binding protein A12 (calgranulin C)
-8.86
0.480
1.00
-2.63
15.19
Enzyme regulator
     MMP14
202827_s_at
matrix metalloproteinase 14 (membrane-inserted)
2.39
0.226
1.00
-1.22
-1.84
     TIMP3
201149_s_at
tissue inhibitor of metalloproteinase 3
2.22
0.467
1.00
-1.71
-3.43
     CASP1
211366_x_at
caspase 1, apoptosis-related cysteine protease
-2.73
0.165
1.00
-1.30
1.01
     MME
203434_s_at
membrane metallo-endopeptidase (CD10)
-3.23
0.476
1.00
-2.35
-1.29
     MMP8
207329_at
matrix metalloproteinase 8 (neutrophil collagenase)
-5.81
0.480
1.00
-2.36
1.69
     RASGRP2
208206_s_at
RAS guanyl releasing protein 2 (calcium and DAG-regulated)
-2.60
0.480
1.00
1.02
3.02
Unknown Function
     CYFIP2
220999_s_at
cytoplasmic FMR1 interacting protein 2
-2.68
0.031
1.00
1.14
1.09

Gene expression fold change (U-F) is presented for unfused sutures (n = 8) compared to fused/fusing sutures (n = 6), with corresponding P-values, and for combined unfused coronal (n = 4) and unfused sagittal (n = 2) sutures compared to unfused lambdoid sutures (n = 2).

Coussens et al. BMC Genomics 2007 8:458   doi:10.1186/1471-2164-8-458