Additional file 1:

Mortality of P. berghei infected mosquitoes of the exposed and non-exposed control lines at 3–4 days after infected blood feeding. Mortality of P. berghei infected mosquitoes of the exposed and non-exposed control lines (from generations 7 to 14) at 3–4 days after infected blood feeding are indicated in the table and graph.

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Additional file 3:

Oocyst numbers in midguts of infected blood-fed mosquitoes from generations 13–16. Numbers of oocysts in control and exposed lines (A and B), of generations 13–16 on day 7 after feeding on P. berghei-infected mice. Total midgut dissected (midguts #), mean and standard error of oocysts numbers (mean ± SE), and p-values from the Mann-Whitney test are presented. Zero oocysts are also included for the calculation of mean oocysts numbers. NS = not significant; S = significant difference.

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Additional file 4:

Genes with higher expression in the control line. Genes with higher expression in the control line as compared to the exposed line. Gene names, their corresponding transcript IDs (ENSANGT numbers), and the average log₂-fold differences are shown (for Gen 13A, Gen 14B, Gen15A, Gen15B, and Gen16A). The fold difference represents the average of the five exposed sets of mosquitoes. The genes are sorted into groups (column C) according to their function (see also Fig. 2 for nomenclature of the group names). CS = cytoskeleton, structure; D = diverse function; DIG = digestive; I = immunity; M = metabolism; RSM = redox, stress, mitochondrial; RTT = replication, transcription, translation; TRP = transport; U = unknown function; D = diverse function. The nomenclature used for the cytochrome P450 and GSTs follows [42].

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Additional file 5:

Genes with higher expression in the exposed lines. Genes with higher expression in the exposed lines as compared to the control line. Gene names, their corresponding transcript IDs (ENSANGT numbers), and the average log₂-fold differences are shown (Gen 13A, Gen 14B, Gen15A, Gen15B, and Gen16A). The fold difference represents the average of the five exposed sets of mosquitoes. The genes are sorted into groups (column C) according to their function (see also Fig. 2 for nomenclature of the group names). CS = cytoskeleton, structure; D = diverse function; DIG = digestive; I = immunity; M = metabolism; RSM = redox, stress, mitochondrial; RTT = replication, transcription, translation; TRP = transport; U = unknown function; D = diverse function. The nomenclature used for the cytochrome P450 follows [42].

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Additional file 6:

Correlation of microarray expression data with real-time qRT-PCR expression data. The log ₂ ratios (exposed/control) of the gene expression from both the microarray and qRT-PCR analyses are presented in the table. The gene name, generation number, and selection line name are listed in column 1. The transcript ID is listed in column 2, with the portion "ENSANGT00000" removed from the ENSEMBL transcript ID.

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Additional file 7:

Real-time quantitative PCR (qRT-PCR) based expression analyses of immune genes in naïve and infected blood-fed mosquitoes (see Fig. 4). This table provides raw data for assays presented in Fig. 4. qRT-PCR expression values for four immune genes (Tep1, LRIM1, SPCLIP1, and IRSP1) from generations 10–13. ^a fold = fold changes of exposed/control (if up-regulated) and control/exposed (if down regulated [25]); ^b S.E. = standard error of three replicates. The transcript IDs of the immune genes are also shown; the ENSANGT00000 portion is shown as E.

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Additional file 2:

Primer sets used for the qRT-PCR in Fig. 3 and Fig. 4. Primer sets used for the qRT-PCR in Fig. 3 and Fig. 4.

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