Table 5

Vector contamination in ESTs sampled from dbEST

Vector

a# Contaminated ESTs

b# ESTs

cRatio


pT7T3D-PacI

20

3883

0.52%

pSPORT1

99

3784

2.62%

pCMVSPORT6.0

17

4784

0.36%

pME18S-FL

13

3382

0.38%

pUC18

17

1618

1.05%

pCS107

3

1198

0.25%

PBRcDNASfiIAB

5

1177

0.42%

pBluescriptSK(-)

20

2494

0.80%

pDNR-LIB

24

1817

1.32%

pOTB7

6

891

0.67%

pBluescriptIISK(+)

27

2516

1.07%

pBK-CMV

11

783

1.40%

pBluescriptSK(+)

11

713

1.54%

pExpress-1

19

1507

1.26%

pBluescriptIIKS(+)

7

689

1.02%

pcDNA3.1(+/-)

8

469

1.71%

pCMV-SPORT6.1

186

1676

11.10%

pTriplEx2

60

545

11.01%

pDONR222

20

516

3.88%

pCS108

1

362

0.28%

pT7T3D

1

559

0.18%


Total

575

35363

1.63%d


aNumber of ESTs which were cloned into the indicated vector remained to be contaminated with vector.

bNumber of ESTs using the indicated vector were subjected to BLAST analysis for identification of vector contamination

cpercentage of vector contamination for the ESTs using the same cloning vector.

dpercetage obtained by 575/35363 = 1.63%

Chen et al. BMC Genomics 2007 8:416   doi:10.1186/1471-2164-8-416

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