Table 2

Cloning vectors, primers and adaptors used for ESTs in this study

Vector (cutting site)

Primer/adaptor

No. seq

Source of EST


PGEM-Teasy (60–61)

primer1, primer2R, adapter1, adapter2R

2882

ABRC laboratories

pTriplEx2 (571–581)

ToTom

2318

ABRC laboratories

pCR21TOPO (294–295)

primer1, primer2R, adapter1, adapter2R

5

ABRC laboratories

pZL1 (239–253)

NotI primer-adapter, SalI adapter

700

ABRC laboratories

pT7Blue (95–96)a

NotI primer-adapter, SalI adapter

130

ABRC laboratories

pCMV-SPORT6.1 (774–1222)

3000

trace archive at NCBI

pT7T3-PAC (200–240)

EcoRI adaptors

3000

trace archive at NCBI

pDNR-LIB (77–298)

Sfi-(dT)30; AAGCAGTGGTATCAACG CAGAGTGGCC

3000

trace archive at NCBI


a Only 14 of the 130 ESTs cloned into pT7Blue in this test used the indicated adapter while the remaining 116 used no adapters.

Chen et al. BMC Genomics 2007 8:416   doi:10.1186/1471-2164-8-416

Open Data