Table 3

Predicted ORFs in the sequence inserted into ORF 32 and their highest BlastX matches. Nucleotide numbering is from the 5' end of the insertion sequence; nucleotide 8685 corresponds to nucleotide 14790 in the BJ1 genomic sequence. The sequence at the site of insertion was tgctcggtcgtcaa/CGACGCCGACGACGGCGA; lower case variant, upper case BJ1 ORF 32. Orfs are in the forward direction with respect to the virus genome unless indicated by a - sign. * indicates a truncated ORF because of incomplete sequencing (V10) or the insertion event itself (V1 and ORF32) aa indicates the number of amino acids.

ORF

Position

Size

Homologs (% Identity)

Start

Stop

(aa)

V10*

2

277

*

67% – ornithine cyclodeaminase Natronomonas pharaonis DSM 2160

V9-

749

351

132

36% – hypothetical protein VNG6157H Halobacterium sp. NRC-1

V8-

1910

843

355

70% – cell division protein pelota Natronomonas pharaonis DSM 2160.

V7-

3051

1936

371

28% – hypothetical protein NP4342A Natronomonas pharaonis

V6

3346

3753

135

38% – hypothetical protein rrnAC2062 Haloarcula marismortui

V5

3912

4685

257

38% – Alpha/beta hydrolase fold protein Ralstonia eutropha JMP134

V4

4747

5058

103

75% – hypothetical protein HQ2797A Haloquadratum walsbyi DSM 16790

V3-

7408

5900

502

73% – RtcB-like protein 1 Natronomonas pharaonis DSM 2160

V2-

7934

7503

143

61% – hypothetical protein NP3986A Natronomonas pharaonis DSM 2160

V1*

8326

8684

119*

64% – 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase) Haloferax volcanii

32*

8685

9059

*

100% Phage BJ1 hypothetical protein

Pagaling et al. BMC Genomics 2007 8:410   doi:10.1186/1471-2164-8-410