Research article

Vascular microarray profiling in two models of hypertension identifies caveolin-1, Rgs2 and Rgs5 as antihypertensive targets

T Hilton Grayson¹ , Stephen J Ohms² , Therese D Brackenbury¹ , Kate R Meaney¹ , Kaiman Peng² , Yvonne E Pittelkow³ , Susan R Wilson³ , Shaun L Sandow⁴ and Caryl E Hill¹

¹Division of Neuroscience, John Curtin School of Medical Research, Australian National University, Canberra, Australia

²Australian Cancer Research Foundation Biomolecular Resource Facility, John Curtin School of Medical Research, Australian National University, Canberra, Australia

³Centre for Bioinformation Science, Mathematical Sciences Institute, Australian National University, Canberra, Australia

⁴School of Medical Sciences, University of New South Wales, Sydney, Australia

author email corresponding author email

BMC Genomics 2007, 8:404doi:10.1186/1471-2164-8-404

Published:	7 November 2007

Abstract

Background

Hypertension is a complex disease with many contributory genetic and environmental factors. We aimed to identify common targets for therapy by gene expression profiling of a resistance artery taken from animals representing two different models of hypertension. We studied gene expression and morphology of a saphenous artery branch in normotensive WKY rats, spontaneously hypertensive rats (SHR) and adrenocorticotropic hormone (ACTH)-induced hypertensive rats.

Results

Differential remodeling of arteries occurred in SHR and ACTH-treated rats, involving changes in both smooth muscle and endothelium. Increased expression of smooth muscle cell growth promoters and decreased expression of growth suppressors confirmed smooth muscle cell proliferation in SHR but not in ACTH. Differential gene expression between arteries from the two hypertensive models extended to the renin-angiotensin system, MAP kinase pathways, mitochondrial activity, lipid metabolism, extracellular matrix and calcium handling. In contrast, arteries from both hypertensive models exhibited significant increases in caveolin-1 expression and decreases in the regulators of G-protein signalling, Rgs2 and Rgs5. Increased protein expression of caveolin-1 and increased incidence of caveolae was found in both smooth muscle and endothelial cells of arteries from both hypertensive models.

Conclusion

We conclude that the majority of differences in gene expression found in the saphenous artery taken from rats with two different forms of hypertension reflect distinctive morphological and physiological alterations. However, changes in common to caveolin-1 expression and G protein signalling, through attenuation of Rgs2 and Rgs5, may contribute to hypertension through augmentation of vasoconstrictor pathways and provide potential targets for common drug development.