Table 1

Summary of mapping resolution for deficiencies using both genetic and molecular mapping techniques.

Genetic/PCR Mapping Data

oaCGH Mapping Data

aSequenced Breakpoint Coordinates


Strain

Deficiency

Mutagen

Position

LEFT Cosmid

RIGHT Cosmid

LEFT (bp)

RIGHT (bp)

LEFT (bp)

RIGHT (bp)

Size of deficiency (kb)


MT3022

nDf20

GRI

Out

egl-5

sma-2

7895240

8678216

7896734

8676211

780

In

lin-36

dpy-19

7900966

8676164

BC4698

sDf121

GRI

Out

F10G11

C05D2

4568979

5637318

4568042

5637312

1069

In

F35G12

F54E7

4569058

5636068

BC4634

sDf125

UVI

Out

F42A10

K04C2

6178564

6620842

6178994

6620106

441

In

C23G10

R13F6

6181034

6619737

BC4638

sDf127

UVI

Out

C23G10

R13A5

6200904

7453868

ND

ND

d1194–1253

In

C23G10

T20B12

6200951

7395215

BC4690

sDf128

GRI

Out

C29E4

C06G4

7945153

8023540

7945392

8023387

78

In

C06G4

F44B9

7945526

8019512

BC4637

sDf130

UV

Out

C32A3

C05D2

3640014

5566628

3640101

5565581

1925

In

3640119

5565449

Out

5635618

5664335

ND

ND

e16–29

In

R13G10

ZC155

5645065

5661035

BC4677

sDf135

GRI

Out

B0361

T20B12

7276848

7479897

7277386

7476402

199

In

T20B12

R13A5

7277471

7467958

bNA

sDp3

GRI

Out

daf-7

dpy-19

cComplex

8611252

NA

NA

NA

In

dpy-18

mig-10

8611166

BC3737

sDp8

UVI

Out

let-721

sma-2

6559307

8714234

NA

NA

NA

In

M01G4

dpy-19

6562088

8710120


Average resolution (kb)

117

5.6

0

0


aBreakpoint coordinates aligned to Wormbase (release WS170).

bsDp3 structure is inferred from the background of the oaCGH data.

cThe left breakpoint of sDp3 is fragmented over a large region.

dThe right breakpoint of sDf127 falls within a 50 kb region of low complexity and therefore low probe density and could not be accurately defined.

eThe second deleted region in sDf130 was not be resolved by PCR analysis.

Jones et al. BMC Genomics 2007 8:402   doi:10.1186/1471-2164-8-402

Open Data