Figure 4.

Verification of selected genes by RT-PCR. The ratio of transcript levels of TNC (Tenascin), FZD8 (Frizzled 8), ADAM12 (ADAM metallopeptidase 12), and CSPG2 (Chondroitin sulfate proteoglycane 2, versican) comparing CAIS cell lines and normal scrotal cell lines. Semi-quantitative RT-PCR was performed on four samples of normal male scrotal fibroblasts (S4, S5, S8, S9), as well as on four samples of labia majora derived from CAIS patients (ARD402, ARD411, ARD682, ARD1097). Differences in expression levels between different cell lines were calculated according to the ΔΔ-CT method [19]. The y-axis represents the ratios of expression levels of CAIS labia majora fibroblasts divided by those from scrotal fibroblasts.