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Table 4
Strains and plasmids used in this study.
Strain	Description	Source

N. gonorrhoeae strains
F62	Parental strain	Laboratory stocks
RUG7001	F62 proAB paniA::lacZ	[43]
RUG7022	F62 proAB paniA::lacZ fnr	[6]
JCGC201	F62 proAB pccp::lacZ	[5]
JCGC202	F62 proAB pccp::lacZ fnr	[5]
JCGC502	F62 fnr-3xFLAG Kan^R	This work
E. coli strains
RV	Parental strain	Laboratory stocks
JCB386	Δ (nirB-cycG) pnirB::lacZ	[44]
JCB3861	JCB386 narXL	This work
JCB3862	JCB386 narXL narQ	This work
JCB3863	JCB386 narXL narQ narP	This work
JCB391	RV narXL narQ pcnB	This work
JCB12	RV narX narQ frdA::lacZ	[10]
Plasmids
pGCFNR3	Gonococcal fnr, under the regulation of the E. coli fnr promoter, cloned into pBR322.	[5]
pGCFNRi	pGCFNR3 with KpnI and XhoI restriction sites engineered downstream of the fnr gene	This work
pGCFNR-FLAG	pGCFNR3 with a 3xFLAG tag and Kanamycin resistance cassette inserted downstream of the fnr gene	This work
pSUB11	Epitope tagging plasmid carrying 3xFLAG tag and kanamycin resistance cassette.	[36]
pGCNarQP	Gonococcal narQP genes under the control of the E. coli fnr promoter cloned into pBR322.	This work
pBADgcQ	Gonococcal narQ gene under the control of the araBAD promoter cloned into pBAD myc-His A.	This work
pBADecQ	E. coli narQ gene under the control of the araBAD promoter cloned into pBAD myc-His A	This work
pRNW18	pBADEcQ containing R54K substitution in narQ	This work
pRNW19	pBADEcQ containing N48E I49E substitution in narQ	This work
pRNW20	pBADEcQ containing N48E I49E R54K substitution in narQ	This work
pRNW29	pBADEcQ containing D43A E45S A46V N48E I49E substitution in narQ	This work
pRNW30	pBADEcQ containing D43A E45S A46V substitution in narQ	This work
pRNW31	pBADEcQ containing D43A E45S A46V R54K substitution in narQ	This work
pRNW32	pBADEcQ containing D43A E45S A46V N48E I49E R54K substitution in narQ	This work
pRNW33	pBADEcQ containing D43A E45S A46V S52N S57A substitution in narQ	This work
pRNW34	pBADEcQ containing D43A E45S A46V N48E I49E S52N R54K S57A substitution in narQ	This work
p7150	E. coli nirB promoter cloned into the lacZ reporter vector pRW50.	[19]
pRNW15	E. coli napF promoter cloned into the lacZ reporter vector pRW50.	This work

Whitehead et al. BMC Genomics 2007 8:35 doi:10.1186/1471-2164-8-35

Table 4