Research article
The small FNR regulon of Neisseria gonorrhoeae: comparison with the larger Escherichia coli FNR regulon and interaction with the NarQ-NarP regulon
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* Corresponding authors: Jeff A Cole j.a.cole@bham.ac.uk - Nigel J Saunders nigel.saunders@path.ox.ac.uk
- † Equal contributors
1 School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
2 The Bacterial Pathogenesis and Functional Genomics Group, The Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
3 The Computational Biology Research Group, The Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
4 The Medical School, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
BMC Genomics 2007, 8:35 doi:10.1186/1471-2164-8-35
Published: 29 January 2007Additional files
Additional file 1:
Primers used in this study. Primer sequences used for construction of plasmids and strains, mutagenesis, and quantitative real-time PCR
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Additional file 2:
Figure of growth curves of JCGC501 and anti FNR-3xFLAG Western blots. Figure showing: A. Growth characteristics of strain JCGC501, carrying a chromosomal fnr-3xFLAG fusion. Strain JCGC501 was grown microaerobically in the presence or absence of 5 mM NaNO2. B. Western blotting shows that the quantity of FNR-3xFLAG protein remains constant through the growth cycle. Samples were taken from the above growth curve at hourly intervals, separated by SDS-PAGE, blotted onto PVDF membrane and FNR-3xFLAG protein was detected using anti-FLAG antibodies and ECF-Plus chemiluminescent labelling.
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