Figure 4.

The percentage coefficient of variation (% CV) for five Arabidopsis thaliana reference genes, from two AtGenExpress experiments [24]. (A) RNA was extracted from leaves 2, 4, 6, 8, 10 and 12, sampled from 17 d old plants (n = 3), labelled and hybridised to the A. thaliana ATH1-121501 GeneChip array, and data were normalised to the median expression value of each gene across all leaves. (B) RNA was extracted from whole rosettes of 7, 14, and 21 d old plants (n = 3), labelled and hybridised to the A. thaliana ATH1-121501 GeneChip array, and normalised to the median expression value of each gene across all time points.