Figure 8.

Quantitative real time RT-PCR analysis of transcriptional levels of selected genes in immune challenged and untreated larvae. The mRNA levels of selected genes in immune challenged animals (black bars) were determined and are shown relative to their expression levels in untreated animals (white bars). The transcriptional rates of eristalin and ET-AMP10 were found to be increased over 14 and 34 fold, respectively, in response to LPS injection. In contrast, the amount of actin and α-tubulin transcripts were not significantly influenced. Results represent mean values of three independent determinations ± S.D.