Quantitative real-time PCR analysis. qRT-PCR was performed on RNAs from 3 different groups of cultured cells to analyze relative mRNA content for 9 key genes: c-Myc, Socs1, Socs 2, Abl1, Jak1, Xbp1, Sdc1, Irf4, Pax5 as well as the "housekeeping gene", GAPDH, using primers and detection oligos designed in consultation with the manufacturer (ABI). The data was presented as mean ± standard deviation values of each group. qRT-PCR data for individual samples are presented as Additional file 3. All data were normalized to GAPDH. These values were then calibrated against the sample with the lowest expression and represented as -ΔΔCt values with standard errors.
Park et al. BMC Genomics 2007 8:302 doi:10.1186/1471-2164-8-302