Figure 2.

Polysomal profiles of wild-type cells and cells expressing rps9-Δ188. Cells expressing only rps9-Δ188 gene (A) or both RPS9 genes (B) were grown to the mid-logarithmic phase and harvested. Cell lysates were separated on a 10%–50% sucrose gradient and the OD254 along the gradient was monitored. The sedimentation position of ribosomal complexes (40S, 60S, 80S and polysomes) is indicated on each panel.