Additional file 1.
Random hexamer versus gene specific priming using highly degraded RNA as a template. The 21 genes from the OncotypeDX Breast Cancer assay were assayed using highly fragmented RNA as template. Random hexamer priming (RH) or gene specific pool (GSP) priming in the reverse transcription reaction were compared. Each gene was run in triplicate wells and the data shown represents the average and standard deviation. The increased specificity of GSP priming allows even the most highly degraded sample to be amplified with robust signal.
Format: DOC Size: 32KB Download file
This file can be viewed with: Microsoft Word Viewer
Clark-Langone et al. BMC Genomics 2007 8:279 doi:10.1186/1471-2164-8-279