Figure 1.

Effect of increasing priming complexity in reverse transcription using high quality RNA template. Eight gene specific primer (GSP) pools each containing from 94 to 96 unique primers were used to prime separate RT-PCR reactions with high quality commercial RNA template. These 8 GSP pools were then combined to make a single GSP pool that was used to prime one RT-PCR reaction using the same template RNA. Both priming methods were performed twice and the average C_T value for each gene was determined for this analysis. The solid line represents the least squares line fit and the dashed line represents the line of concordance.