Table 6

PRI-lock assay validation. Analysis of genomic DNA targets mixed in different ratios.

Genomic DNA template (pg/μl ligation mixture)

Log10 target (copy number/μl ligation mixture) (SD)



F. oxysporum

P. fragariae

M. roridum

R. solani AG 4-1

R. solani AG 4-2

E. c. c.

F. oxysporum

P. fragariae

M. roridum

R. solani AG 4-1

R. solani AG 4-2

E. c. c.

Gamma Proteo bacterial spp.



100

100

100

--

--

10

6.3 (0.10)

6.8 (0.08)

6.8 (0.07)

--

--

3.5 (0.09)

4.6 (0.10)

1

0.1

100

--

--

--

4.2 (0.12)

3.6 (0.27)

6.7 (0.05)

--

--

--

--

100

0.01

0.01

--

--

100

6.1 (0.14)

nd

nd

--

--

4.4 (0.08)

5.5 (0.13)

10

100

1

--

--

1

5.1 (0.08)

6.8 (0.09)

4.4 (0.11)

--

--

2.8 (0.17)

3.5 (0.11)

10

0.01

1

--

--

10

5.2 (0.17)

nd

4.5 (0.04)

--

--

3.5 (0.20)

4.5 (0.16)

10

0.001

1

--

--

0.01

5.1 (0.09)

nd

4.4 (0.20)

--

--

nd

nd

10

1

0.1

--

--

100

5.1 (0.15)

4.6 (0.14)

3.3 (0.04)

--

--

4.4 (0.07)

5.4 (0.05)

10

10

10

10

10

10

5.0 (0.07)

5.6 (0.05)

5.4 (0.06)

4.3 (0.08)

5.0 (0.20)

3.2 (0.23)

4.3 (0.08)

--

--

--

100

1

--

--

--

--

5.7 (0.06)

4.0 (0.23)

--

--

0.1

--

--

1

--

10

3.8 (0.20)

--

--

3.3 (0.16)

--

3.5 (0.10)

4.6 (0.10)


Target numbers were calculated using the CT values and the corresponding calibration curves. Data highlighted in bold font, indicate data outside the linear range. Data represent 2 ligation replicates (n = 2), each with 4 qPCR replicates (n = 4). Nd: not detectable. E.c.c.:Erwinia carotovora carotovora

van Doorn et al. BMC Genomics 2007 8:276   doi:10.1186/1471-2164-8-276

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