Table 4

Quantification and sensitivity of the developed assay on the Biotrove OpenArray™ platform.

PRI-lock probe

Calibration curve formula

R2 value

Detection limit (copy number/μl ligation mixture)

Linear quantification range (copy number/μl ligation mixture)


Phytophthora spp.

y = -3.61x + 38.1

0.995

104

108 – 104

P. infestans

y = -3.31x + 35.7

0.989

103

108 – 103

R. solani AG 2-2

y = -3.49x + 37.9

0.994

104

108 – 104

R. solani AG 4-1

y = -3.49x + 35.8

0.994

103

109 – 103

R. solani AG 4-2

y = -3.35x + 39.2

0.998

103

108 – 104

F. oxysporum

y = -3.47x + 39.1

0.990

104

108 – 104

M. roridum

y = -3.15x + 36.6

0.984

104

109 – 104

V. dahliae

y = -3.42x + 36.3

0.996

103

108 – 103

V. alboatrum/V. tricorpus

y = -3.40x + 38.8

0.991

103

108 – 104

M. hapla

y = -3.39x + 38.1

0.989

104

108 – 104

E. carotovora carotovora

y = -3.51x + 36.3

0.997

103

109 – 103

A. tumefaciens

y = -3.36x + 36.5

0.995

103

108 – 103

G. Proteo bacterial spp

y = -3.51x + 38.1

0.995

104

108 – 104


(x) = Log10(copy number target input/μl ligation mixture). (y) = CT value.

van Doorn et al. BMC Genomics 2007 8:276   doi:10.1186/1471-2164-8-276

Open Data