Table 3

Specificity of the PRI-lock probe system in conventional real-time PCR and in the Biotrove OpenArray™ for non-target organisms with closely related ligation sites.

Pythium undulatum cnt Phytophthora spp.(1)

Phytophthora sojae cnt P. infestans (3)

Myrothecium verrucaria cnt M. roridum (5)

PRI-lock Probe

CT(AB)

CT(BT)

CT(AB)

CT(BT)

CT(AB)

CT(BT)


Phytophthora spp.

nd

nd

15.5 (0.11)

16.1 (0.10)

nd

nd

P. infestans

nd

nd

nd

nd

nd

nd

M. roridum

nd

nd

nd

nd

nd

nd


The PRI-lock probes were ligated on 106 targets/μl ligation mixture. CT values were normalized using the ILC control PRI-lock probe. Data represent average CT values of three PCR replicates in the conventional real-time platform (n = 3) and of four PCR replicates in the Biotrove OpenArray™ (n = 4). Standard deviations are indicated between brackets. AB: samples run on the conventional real-time PCR platform. BT: Samples tested on the Biotrove OpenArray™ platform. Number of discriminating nucleotides in the ligation region are indicated between brackets. nd: not detectable. cnt: closest non target.

van Doorn et al. BMC Genomics 2007 8:276   doi:10.1186/1471-2164-8-276

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