Table 2

Changes observed in the post-translational modification of proteins as a result of bldA mutation

Gene

Function1

Alteration in protein abundance2 observed for:

Spot 1

Spot 2


    A) Spots differ in isoelectric point (pI): spot 1 has more acidic pI than spot 2

SCO1246

BioD (dethiobiotin synthetase)

>2-fold up in ΔbldA

>2-fold down in ΔbldA

SCO1916

Putative transferase

Absent in ΔbldA

No change

SCO2390

FabF (3-oxoacyl- [ACP] synthase II

No change

>2-fold down in ΔbldA

SCO2618

ClpP2 (Clp-protease subunit 2)

>2-fold up in ΔbldA

>2-fold down in ΔbldA

SCO3073

HutU (urocanate hydratase)

No change

Absent in ΔbldA

SCO3137

GalE1 (UDP-glucose 4-epimerase)

Absent in ΔbldA

No change

SCO3409

Ppa (inorganic pyrophosphatase)

>2-fold up in ΔbldA

>2-fold down in ΔbldA

SCO4164

CysA (thiosulphate supfurtransferase)

>2-fold up in ΔbldA

>2-fold down in ΔbldA

SCO7400

ABC transport protein

>2-fold down in ΔbldA

No change

    B) Spots differ in Mwt (and pI): spot 1 has higher Mwt than spot 2

SCO2271

Putative membrane protein

>2-fold down in ΔbldA

No change

SCO5465

Conserved hypothetical protein

Absent in ΔbldA

>2-fold up in ΔbldA


1from ScoDB [55]

2protein abundance profiles for all 22 protein spots are detailed in Additional File 7.

Hesketh et al. BMC Genomics 2007 8:261   doi:10.1186/1471-2164-8-261

Open Data