Table 2

Comparison of VSG N-terminal domains of field VSGs with genomic VSG sequences
database name
 Tbb gene with greatest identity
 NTD ORF intact?
 identity (%)
 Tbg gene with greatest identity
 NTD ORF intact?
 identity (%)
 difference in % identities
VSG Ako 1
 contig 11608
 No
 73
 gamb276c12.p1k_3
 Yes
 84
 11
VSG Ako 2
 Tb11.24.0011
 Yes
 79
 gamb843f08.p1k
 No
 79
 0
VSG Bug 1
 Tb927.5.190
 Yes
 58
 gamb1660g03.q1k H
 Yes
 50
 8
VSG Bug 2
 -
 -
 -
 gamb856d03.q1k_1
 Yes
 93
VSG Buteba 1
 Tb10.v04.0152
 Yes
 69
 gamb333c09.p1k H
 partial
 67
 2
VSG Buteba 2
 -
 -
 -
 gamb1441g08.p1k H
 partial
 84
VSG Buteba 3
 Tb09.142.0130
 No
 84
 gamb1420g06.q1k H
 Yes
 92
 8
VSG Buteba 4
 Tb927.3.390
 No
 67
 chr3
 No
 74
 7
VSG Buteba 6
 Tb927_11_02_v4
 Yes
 46
 -
 -
 -
VSG Buw 1
 Tb09.244.0090
 No
 80
 gamb217f04.p1k
 No
 69
 11
VSG Buw 2
 Tb05.26C7.320
 No
 74
 -
 -
 -
VSG Do 1
 Tb09.244.1110
 Yes
 45
 gamb261a02.p1k
 Yes
 44
 1
VSG Do 2
 tryp_XI-346e09.p1k
 No
 64
 -
 -
 -
VSG Kinu 1
 Tb09.244.0250
 Yes
 95
 gamb606g06.q1k H
 -
 93
 2
VSG Maw-ero 1
 -
 -
 -
 gamb1327e07.q1k
 partial
 42
VSG Mul 1
 -
 -
 -
 -
 -
 -
VSG Mul 3
 Tb10.v4.0093
 No
 83
 gamb86e09.p1k H
 No
 85
 2
VSG Mul 4
 Tb09.244.1190
 Yes
 80
 gamb248h03.p1k H
 No
 72
 8
Average

71

73

The N-terminal domains of the VSGs expressed by field isolates have similar percentage identities to homologues from T. b. brucei as to homologues from T. b. gambiense. The 'NTD ORF intact?' column indicates whether the genomic homologue encoded an N-terminal domain that was apparently functional and the percentage identity is over the whole N-terminal domain or all the available sequence (see Supplementary Material).

Hutchinson et al. BMC Genomics 2007 8:234   doi:10.1186/1471-2164-8-234