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Representative Bvg+ phase specific genes identified by microarray analysis. |
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| Function |
Gene |
Product |
Fold-Change ± SEM |
|
|
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| Cell Adhesin |
BB0110 |
putative adhesion |
8.58 ± 3.21 |
| BB1658 fim3 |
serotype 3 fimbrial subunit precursor |
32.47 ± 11.10 |
|
| BB1936 fhaL |
adhesion |
8.04 ± 1.63 |
|
| BB2312 fhaS |
adhesion |
10.37 ± 2.76 |
|
| BB2989 fimD |
fimbrial adhesion |
11.58 ± 3.96 |
|
| BB2992 fimA |
fimbrial protein |
33.75 ± 13.88 |
|
| BB2993 fhaB |
filamentous hemagglutin |
11.37 ± 4.55 |
|
| BB3424 |
fimbrial protein |
8.92 ± 2.64 |
|
| BB3425 fimN |
fimbrial subunit protein |
11.95 ± 2.06 |
|
| BB3426 fimX |
fimbrial protein |
8.92 ± 2.64 |
|
| BB3674 fim2 |
serotype 2 fimbrial subunit precursor |
21.89 ± 11.68 |
|
| Cell Envelope |
BB1289 |
putative integral membrane protein |
26.29 ± 8.25 |
| BB1368 |
putative membrane protein |
3.94 ± 0.56 |
|
| BB3119 |
putative membrane protein |
19.27 ± 2.17 |
|
| BB3157 |
putative membrane protein |
16.41 ± 5.54 |
|
| BB4029 |
putative glycosyl transferase |
4.96 ± 0.32 |
|
| BB4266 |
putative membrane protein |
6.84 ± 0.82 |
|
| BB4269 |
putative glycosyl transferase |
2.23 ± 0.42 |
|
| BB4284 |
putative membrane protein |
2.96 ± 0.14 |
|
| Protein Folding and Ushering |
BB2320 dsbG |
thiol:disulfide interchange protein precursor |
35.29 ± 9.68 |
| BB2991 fimB |
chaperone protein |
11.20 ± 7.71 |
|
| BB3749 mucD |
serine protease; trypsin activity |
2.75 ± 0.41 |
|
| BB3803 |
putative peptidyl-prolyl cis-trans isomerase |
4.65 ± 1.26 |
|
| Global Regulatory Functions |
BB0308 |
putative transcriptional regulator |
5.67 ± 0.98 |
| BB1607 |
putative LysR-family transcriptional regulator |
3.37 ± 0.55 |
|
| BB1638 brpL |
putative RNA polymerase sigma factor |
50.66 ± 15.58 |
|
| BB1642 |
putative regulator; catalytic activity |
112.16 ± 97.97 |
|
| BB1645 |
putative anti-sigma factor; ATP binding |
9.46 ± 3.00 |
|
| BB1646 |
putative antisigma factor antagonist |
18.19 ± 6.08 |
|
| BB2051 |
putative regulatory protein |
2.24 ± 0.59 |
|
| BB4374 |
putative AsnC-family transcriptional regulator |
5.78 ± 7.25 |
|
| Metabolism |
BB0457 |
probable enoyl-CoA hydrates; isomerase activity |
5.72 ± 1.34 |
| BB0458 |
probable carboxymuconolactone decarboxylase |
3.42 ± 0.79 |
|
| BB1476 |
putative gluconate dehydrogenase |
2.88 ± 0.51 |
|
| BB1478 |
putative carbonic anhydrase precursor |
4.05 ± 0.77 |
|
| BB1640 |
hypothetical protein; glutamate-cysteine ligase activity |
63.92 ± 18.15 |
|
| BB1644 alr |
alanine racemase, catabolic |
253.96 ± 97.47 |
|
| BB2658 |
glutamate decarboxylase |
2.78 ± 0.93 |
|
| BB3783 |
intracellular PHB depolymerase |
3.23 ± 0.78 |
|
| BB4286 nadC |
putative nicotinate-nucleotidepyrophosphorylase |
32.86 ± 6.48 |
|
| Periplasmic, Exported, Lipoprotein |
BB0324 cyaA |
bifunctional hemolysin-adenylatecyclase precursor |
6.73 ± 2.52 |
| BB1280 |
putative exported protein |
73.44 ± 37.80 |
|
| BB1292 |
putative exported protein |
272.88 ± 93.88 |
|
| BB1641 |
putative exported protein |
149.05 ± 97.51 |
|
| BB2398 |
putative exported protein |
6.77 ± 1.38 |
|
| BB2873 |
putative membrane protein |
26.85 ± 13.90 |
|
| BB3068 |
putative exported protein |
10.90 ± 3.11 |
|
| BB3242 |
putative exported protein |
48.75 ± 9.65 |
|
| BB3302 |
lipoprotein |
5.09 ± 0.63 |
|
| BB3978 dnt |
dermonecrotic toxin |
8.63 ± 3.61 |
|
| BB4181 |
putative lipoprotein |
3.40 ± 0.25 |
|
| BB4285 |
putative exported protein |
49.73 ± 14.04 |
|
| Transport |
BB0323 cyaC |
cyclolysin-activating lysine-acyltransferase |
2.70 ± 0.27 |
| BB0325 cyaB |
cyclolysin secretion ATP-binding protein; ABC transporter |
2.10 ± 0.31 |
|
| BB0326 cyaD |
cyclolysin secretion protein; transporter activity |
3.48 ± 0.51 |
|
| BB0327 cyaE |
cyclolysin secretion protein; subtilase activity |
3.66 ± 1.57 |
|
| BB0419 sphB1 |
autotransporter subtilisn-like protease; substilase activity |
59.34 ± 43.26 |
|
| BB1593 |
putative ABC transport system, membrane protein |
83.60 ± 28.88 |
|
| BB1643 |
putative inner membrane transport protein |
39.61 ± 16.68 |
|
| BB1864 vag8 |
autotransporter |
122.00 ± 79.14 |
|
| BB2270 |
autotransporter |
4.71 ± 2.07 |
|
| BB3826 bfrD |
probable TonB-dependent receptor for iron transport |
2.68 ± 0.58 |
|
| BB3993 ompQ |
outer membrane porin protein OmpQ |
24.34 ± 8.07 |
|
| TTSS |
BB1616 bopN |
putative outer protein N |
504.05 ± 189.58 |
| BB1620 bopD |
putative outer protein D |
457.16 ± 172.40 |
|
| BB1624 bscI |
putative type III secretion protein |
226.35 ± 80.58 |
|
| BB1628 bscN |
putative ATP synthase in type III secretion |
115.85 ± 37.00 |
|
| BB4228 bopCa |
hypothetical protein; characterized type III effector |
15.70 ± 3.85 |
|
|
DNA microarray analysis was used to measure mRNA levels present in B. bronchiseptica RB50 compared to mRNA levels present in B. bronchiseptica RB54. Differences in mRNA levels are listed as mean fold-changes ± standard error. Fold-changes were calculated by averaging the data from three biological sample sets. aRecently characterized [24]. | |||
Nicholson BMC Genomics 2007 8:220 doi:10.1186/1471-2164-8-220 |
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