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Open Access Methodology article

Using biomarker signature patterns for an mRNA molecular diagnostic of mouse embryonic stem cell differentiation state

Daniel YL Yap1*, David K Smith2, Xue W Zhang3 and Jeffrey Hill1

Author Affiliations

1 Bioinformatics Institute, 30 Biopolis Street, #07-01, Matrix, 138671, Singapore

2 Department of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China

3 College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, Guangdong, China

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BMC Genomics 2007, 8:210  doi:10.1186/1471-2164-8-210

Published: 3 July 2007

Abstract

Background

The pluripotency and self-renewal capabilities, which define the "stemness" state, of mouse embryonic stem (ES) cells, are usually investigated by functional assays or quantitative measurements of the expression levels of known ES cell markers. Strong correlations between these expression levels and functional assays, particularly at the early stage of cell differentiation, have usually not been observed. An effective molecular diagnostic to properly identify the differentiation state of mouse ES cells, prior to further experimentation, is needed.

Results

A novel molecular pattern recognition procedure has been developed to diagnose the differentiation state of ES cells. This is based on mRNA transcript levels of genes differentially expressed between ES cells and their differentiating progeny. Large publicly available ES cell data sets from various platforms were used to develop and test the diagnostic model. Signature patterns consisting of five gene expression levels achieved high accuracy at determining the cell state (sensitivity and specificity > 97%).

Conclusion

The effective ES cell state diagnostic scheme described here can be implemented easily to assist researchers in identifying the differentiation state of their cultures. It also provides a step towards standardization of experiments relying on cells being in the stem cell or differentiating state.