BMC Genomics

official impact factor 4.21

Open Access Research article

Development of the first marmoset-specific DNA microarray (EUMAMA): a new genetic tool for large-scale expression profiling in a non-human primate

Nicole A Datson1*, Maarten C Morsink1, Srebrena Atanasova2, Victor W Armstrong2, Hans Zischler3, Christina Schlumbohm4, Bas E Dutilh5, Martijn A Huynen5, Brigitte Waegele6, Andreas Ruepp6, E Ronald de Kloet1 and Eberhard Fuchs4

Author Affiliations

1 Division of Medical Pharmacology, Leiden/Amsterdam Center for Drug Research and Leiden University Medical Center, The Netherlands

2 Department of Clinical Chemistry, Georg-August University, Goettingen, Germany

3 Institute of Anthropology, University of Mainz, Mainz, Germany

4 Clinical Neurobiology Laboratory, German Primate Center, Göttingen, Germany

5 Center for Molecular and Biomolecular Informatics/Nijmegen Center for Molecular Life Sciences, Radboud University Nijmegen, Geert Grooteplein 28, 6525 GA, Nijmegen, The Netherlands

6 Institute for Bioinformatics, GSF – National Research Center for Environment and Health, Ingolstaedter Landstrasse 1, Germany

For all author emails, please log on.

BMC Genomics 2007, 8:190 doi:10.1186/1471-2164-8-190

Published: 25 June 2007

Additional files

Additional file 1:

Gene content of the marmoset microarray and Gene Ontology Classification. Excel file on content of the marmoset array. From left to right are columns indicating the Probe set ID on the marmoset microarray, the corresponding gene symbol (if known) and gene symbol aliases as well as the gene title. In the next column the source of the sequence is indicated, meaning whether it is derived from the set of 3215 ESTs or an already existing GenBank accession or if is a control sequence. Subsequently the GenBank accessions of the marmoset sequences and the gene ontology classification according to biological process, molecular function and cellular compartment, pathway and if applicable a pathway hyperlink are indicated.

Format: XLS Size: 796KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

Overview of tissue-specific expression profiles. Excel file showing hybridisation signals and detection calls for all tissues tested on the array. From left to right the Probe set ID on the marmoset array, the corresponding gene symbol (if known) and gene symbol aliases as well as the gene title are indicated. In the next columns the signals and the corresponding detection calls are listed for each tissues and finally a string of all detection calls is listed to facilitate identification of transcripts with a tissue-specific pattern of expression.

Format: XLS Size: 1.2MB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 2:

Overview of 3215 sequences submitted to GenBank and dbEST. Excel file giving a detailed overview of the panel of 3215 marmoset ESTs. From left to right the columns indicate the name of the EST clone, the corresponding gene symbol and aliases. The column "source" indicates if the sequence was mappable to a genome, could be assigned a gene name or not or contained a (partial) ORF. The next columns indicate the GenBank Accession number and whether the sequence was submitted to GenBank of dbEST. Finally, the last 2 columns indicate whether the sequence is represented on the marmoset array and if so by which probe set(s).

Format: XLS Size: 554KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data