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Open Access Research article

Ancestral European roots of Helicobacter pylori in India

S Manjulata Devi1, Irshad Ahmed23, Paolo Francalacci4, M Abid Hussain1, Yusuf Akhter1, Ayesha Alvi1, Leonardo A Sechi56, Francis Mégraud57 and Niyaz Ahmed15*

Author Affiliations

1 Pathogen Evolution Group, Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India

2 Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences and allied Hospitals, Hyderabad, India

3 Department of Microbiology, Shri Shivaji College of Arts, Commerce and Science (SGB Amravati University), Akola, MS, India

4 Dipartimento di Zoologia e Genetica Evoluzionistica, University of Sassari, Sassari, Italy

5 ISOGEM Collaborative Network on Genetics of Helicobacters (The International Society for Genomic and Evolutionary Microbiology, University of Sassari, Sassari, Italy)

6 Dipartimento de Scienze Biomediche, University of Sassari, Sassari, Italy

7 INSERM U853 and Centre National de Référence des Campylobacters et Hélicobacters, Laboratoire de Bactériologie, Université Victor Segalen Bordeaux 2, France

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BMC Genomics 2007, 8:184  doi:10.1186/1471-2164-8-184

Published: 20 June 2007

Abstract

Background

The human gastric pathogen Helicobacter pylori is co-evolved with its host and therefore, origins and expansion of multiple populations and sub populations of H. pylori mirror ancient human migrations. Ancestral origins of H. pylori in the vast Indian subcontinent are debatable. It is not clear how different waves of human migrations in South Asia shaped the population structure of H. pylori. We tried to address these issues through mapping genetic origins of present day H. pylori in India and their genomic comparison with hundreds of isolates from different geographic regions.

Results

We attempted to dissect genetic identity of strains by multilocus sequence typing (MLST) of the 7 housekeeping genes (atpA, efp, ureI, ppa, mutY, trpC, yphC) and phylogeographic analysis of haplotypes using MEGA and NETWORK software while incorporating DNA sequences and genotyping data of whole cag pathogenicity-islands (cagPAI). The distribution of cagPAI genes within these strains was analyzed by using PCR and the geographic type of cagA phosphorylation motif EPIYA was determined by gene sequencing. All the isolates analyzed revealed European ancestry and belonged to H. pylori sub-population, hpEurope. The cagPAI harbored by Indian strains revealed European features upon PCR based analysis and whole PAI sequencing.

Conclusion

These observations suggest that H. pylori strains in India share ancestral origins with their European counterparts. Further, non-existence of other sub-populations such as hpAfrica and hpEastAsia, at least in our collection of isolates, suggest that the hpEurope strains enjoyed a special fitness advantage in Indian stomachs to out-compete any endogenous strains. These results also might support hypotheses related to gene flow in India through Indo-Aryans and arrival of Neolithic practices and languages from the Fertile Crescent.