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Open Access Research article

An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice

Chen Cheng1, Kil-Young Yun1, Habtom W Ressom2, Bijayalaxmi Mohanty3, Vladimir B Bajic4, Yulin Jia5, Song Joong Yun6 and Benildo G de los Reyes1*

Author Affiliations

1 Department of Biological Sciences, University of Maine, Orono, ME 04469, USA

2 Lombardi Comprehensive Cancer Research Center, Georgetown University, Washington, DC 20057, USA

3 Institute for Infocomm Research, 21 Heng Mui Keng Terrace, 119613, Singapore

4 South African National Bioinformatics Institute, University of the Western Cape, Bellville, 7535, South Africa

5 USDA-ARS, Dale Bumpers National Rice Research Center, Stuttgart, AR 72160, USA

6 Division of Biological Resources Sciences and Institute of Agricultural Science and Technology, Chonbuk National University, Jeonju 561-756, South Korea

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BMC Genomics 2007, 8:175  doi:10.1186/1471-2164-8-175

Published: 18 June 2007

Abstract

Background

Plants respond to low temperature through an intricately coordinated transcriptional network. The CBF/DREB-regulated network of genes has been shown to play a prominent role in freeze-tolerance of Arabidopsis through the process of cold acclimation (CA). Recent evidence also showed that the CBF/DREB regulon is not unique to CA but evolutionarily conserved between chilling-insensitive (temperate) and chilling-sensitive (warm-season) plants. In this study, the wide contrast in chilling sensitivity between indica and japonica rice was used as model to identify other regulatory clusters by integrative analysis of promoter architecture (ab initio) and gene expression profiles.

Results

Transcriptome analysis in chilling tolerant japonica rice identified a subset of 121 'early response' genes that were upregulated during the initial 24 hours at 10°C. Among this group were four transcription factors including ROS-bZIP1 and another larger sub-group with a common feature of having as1/ocs-like elements in their promoters. Cold-induction of ROS-bZIP1 preceded the induction of as1/ocs-like element-containing genes and they were also induced by exogenous H2O2 at ambient temperature. Coordinated expression patterns and similar promoter architectures among the 'early response' genes suggest that they belong to a potential regulon (ROS-bZIP – as1/ocs regulatory module) that responds to elevated levels of ROS during chilling stress. Cultivar-specific expression signatures of the candidate genes indicate a positive correlation between the activity of the putative regulon and genotypic variation in chilling tolerance.

Conclusion

A hypothetical model of an ROS-mediated regulon (ROS-bZIP – as1/ocs) triggered by chilling stress was assembled in rice. Based on the current results, it appears that this regulon is independent of ABA and CBF/DREB, and that its activation has an important contribution in configuring the rapid responses of rice seedlings to chilling stress.