Open Access Highly Accessed Research article

A global view of Staphylococcus aureus whole genome expression upon internalization in human epithelial cells

Christian Garzoni1, Patrice Francois1*, Antoine Huyghe1, Sabine Couzinet1, Caroline Tapparel1, Yvan Charbonnier1, Adriana Renzoni1, Sacha Lucchini2, Daniel P Lew1, Pierre Vaudaux1, William L Kelley1 and Jacques Schrenzel1

Author Affiliations

1 Service of Infectious Diseases, University Hospital of Geneva, Department of Internal Medicine, 24 rue Micheli-du-Crest, CH-1211 Geneva 14, Switzerland

2 Molecular Microbiology Group, Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK

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BMC Genomics 2007, 8:171  doi:10.1186/1471-2164-8-171

Published: 14 June 2007

Additional files

Additional file 1:

Reproducibility of RNA amplification. cDNA was generated in 2 separate reactions from the same RNA pool, amplified independently and hybridized in 2 different arrays. Labeled cDNA from the starting material was used as control. Dye normalized intensities of amplified material are plotted in both axis.

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Additional file 2:

Fold changes at 2 h and 6 h of all genes compared to control bacteria. Values represent mean of fold change from 3 or 4 independent biological replicates. * Some genes are classified in two different COG categories and appear on separate lines in the table.

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Additional file 3:

Genes differentially expressed grouped by function. Values represent mean of fold change from 3 or 4 independent biological replicates. * Some genes are classified in two different COG categories and appear on separate lines in the table.

Format: PDF Size: 38KB Download file

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Open Data