Table 2

Confirmation of microarray results via real time PCR.

Gene accession

Gene name

Microarray data

Real time PCR data


Signal intensity (pixel)

relative expression

relative expression


High expression


actin

33512

1

1

XM_645064

ehcp-a1

17691

0.528

0.853

XM_645550

ehcp-a2

32474

0.969

0.853

XM_645845

ehcp-a5

8628

0.257

0.368


Intermediate


XM_648987

ehasp22-1

2156

0.064

0.020

XM_652272

ehcp-a6

802

0.024

0.026

XM_650130

ehsp 9-2

974

0.029

0.035

XM_651453

XM_650504

XM_647071

ehmp20-3

2838

0.085

0.360

XM_651374

ehmp48-1

895

0.027

0.020


Low


XM_648162

ehcp-a3

549

0.016

0.000

XM_651510

ehcp-a4

414

0.012

0.013

XM_652354

ehcp-a8

510

0.015

0.010

XM_647728

ehasp 22-2

458

0.014

0.000

XM_652471

ehasp 22-3

450

0.013

0.010

XM_648604

ehasp 22-4

516

0.015

0.000

XM_651670

ehsp28-1

380

0.011

0.010

XM_646997

ehsp 28-4

525

0.016

0.020

XM_650302

ehmp8-1

542

0.016

0.015

XM_647540

ehmp8-2

686

0.020

0.010

XM_649181

ehmp49-1

406

0.012

0.023

XM_647901

ehcp-b9

144

0.004

0.000

XM_651336

ehmp20-1

282

0.008

0.000

XM_643899

ehsp 28-2

230

0.007

0.009


Gene expression profile of selected peptidases in E. histolytica isolate HM-1:IMSS.

For microarray analysis nine independent experiments were performed. The signal intensity is indicated in pixel. For real time PCR analysis all primer sets were run in duplicates. Two biological replicates were investigated. The expression of actin as normalizer was set to 1. The relative expression of the peptidases were related to actin.

Tillack et al. BMC Genomics 2007 8:170   doi:10.1186/1471-2164-8-170

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