A flow chart outlines the search strategy used to identify candidate transcription factors that might be associated with the tissue-specific expression of miRNAs in the most differentially expressed groups (A). (B) A screen shot of the "regulogram" map from GenomeTraFac showed a peak for the "Hits" (red circle) upstream to the hsa-miR-129-2 (miR-129b) locus. (C) A screen shot of the transcription factor binding mapfrom GenomeTraFac showed four transcription factors with matching tissue distribution with that of hsa-miR-129b. STAT, STAT5; INSM, INSM1; SORY, SOX5; MOKF, MOK-2 (ZFP239). (D) A screen shot from the GNF database showed the expression of MOK-2 (ZFP239) in normal mouse tissues (no data availablefor human tissues). Two screen shots of the "regulogram" map from GenomeTraFac showed two regions of genomic sequences (red circles) close tothe hsa-miR-302b (E) and hsa-miR-34b (F) loci from which the binding sites for Nkx2-5 and FOXF2, respectively, were identified.
Liang et al. BMC Genomics 2007 8:166 doi:10.1186/1471-2164-8-166