Table 1

Putative genes idenitified in cDNA clones of Puccinia striiformis f. sp. tritici based on their sequence comparison with other fungal genes through Blastx search of the NCBI databases

Category & clone no.

GenBank accession

Size (bp)

Full length or partiala

Best hit in the NCBI fungal databases


Protein

Accession

Organism

e-value


1. Amino acid metabolism

65N4

EG374380

2044

F

Glycine hydroxymethyltransferase

gb|AAW45780.1

Cryptococcus neoformans

1.00E-156

60J18a

EG374421

1142

P

Potential kynurenine 3-monooxygenase

gb|EAK98864.1

Candida albicans

2.00E-06

60J18b

EG374422

1220

P

Potential kynurenine 3-monooxygenase

gb|EAK98864.1

Candida albicans

1.00E-12

58D15a

EG374299

897

P

Saccharopine dehydrogenase

gi|70993695

Aspergillus fumigatus

2.00E-55

58D15b

EG374300

780

P

Spermidine synthase

emb|CAD71251.1

Neurospora crassa

3.00E-78

2. Cell Defense

35A16

EG374447

1351

F

Related to stress response protein

emb|CAD21425.1

Neurospora crassa

2.00E-23

3. Cell division/cycle

80F12

EG374389

1560

F

Cell division control protein

gb|AAB69764.1

Candida albicans

2.00E-28

65O23

EG374383

2037

F

Cyclin c homolog 1

ref|NP_596149.1

Schizosaccharomyces pombe

3.00E-07

4. Cell signaling/cell communication

40D3

EG374466

1534

F

Autophagy-related protein

gb|AAW43831.1

Cryptococcus neoformans

6.00E-45

70C17a

EG374441

1206

P

Fasciclin I family protein

gi|44890027

Aspergillus fumigatus

3.00E-06

58J15b

EG374311

807

P

GTPase activating protein

gb|AAW43777.1

Cryptococcus neoformans

2.00E-09

55B10a

EG374277

861

P

MAP kinase 1

gb|AAO61669.1

Cryptococcus neoformans

3.00E-19

55B10b

EG374278

932

P

MAP kinase

gb|AAU11317.1

Alternaria brassicicola

7.00E-74

65M20

EG374379

1098

F

Nucleoside-diphosphate kinase

emb|CAD37041.1

Neurospora crassa

9.00E-53

70E5

EG374404

1766

F

Serine/threonine kinase

gi|58262703

Cryptococcus neoformans

3.00E-61

10D13a

EG374414

1122

P

Serine palmitoyl transferase subunit

gb|AAP47107.1

Aspergillus nidulans

4.00E-27

10D13b

EG374416

1170

P

Serine palmitoyl transferase subunit

gb|AAP47107.1

Aspergillus nidulans

2.00E-18

30G12

EG374337

1131

F

Signal peptidase 18 KD subunit

emb|CAE76335.1

Neurospora crassa

3.00E-10

5. Cell Structure and growth

58H22a

EG374306

920

P

Beta-tubulin

emb|CAC83953.1

Uromyces viciae-fabae

3.00E-72

58H22b

EG374307

859

P

Beta-tubulin

emb|CAC83953.1

Uromyces viciae-fabae

5.00E-68

10I12

EG374325

1105

F

Conidiation protein 6

emb|CAD70456.1

Neurospora crassa

2.00E-10

30J9

EG374343

1302

F

Deacetylase

emb|CAD10036.1

Cryptococcus neoformans

2.00E-43

60C15

EG374348

1456

F

Deacetylase

gb|AAW47023.1

Cryptococcus neoformans

6.00E-35

65D17

EG374372

1449

F

Deacetylase

emb|CAD10036.1

Cryptococcus neoformans

4.00E-36

40F18

EG374469

1117

F

Deacetylase

emb|CAD10036.1

Cryptococcus neoformans

2.00E-31

55D17

EG374475

1619

F

Deacetylase

emb|CAD10036.1

Cryptococcus neoformans

5.00E-18

35C19b

EG374494

836

P

Deacetylase

emb|CAD10036.1

Cryptococcus neoformans

6.00E-18

10C3

EG374321

1479

F

Deacetylase

gb|AAW47023.1

Cryptococcus neoformans

6.00E-26

35N24

EG374461

783

F

Hydrophobin

emb|CAD42710.1

Davidiella tassiana

5.00E-34

32H21a

EG374436

1176

P

Intraorganellar peroxisomal translocation component Pay32p (PAY32) gene

gi|5821763

Yarrowia lipolytica

4.00E-32

40B22

EG374465

1708

F

Nuclear filament-containing protein

emb|CAA93293.1|

Schizosaccharomyces pombe

5.00E-16

35G11a

EG374497

819

P

Pria_lened pria protein

emb|CAA43289.1

Lentinula edodes

2.00E-12

65M2

EG374413

2097

F

UDP-glucose dehydrogenase

gb|AAS20528.1

Cryptococcus neoformans

1.00E-145

6. Energy/TCA cycle

35D23b

EG374496

629

P

64 kDa mitochondrial NADH dehydrogenase

gb|AAW44492.1

Cryptococcus neoformans

1.00E-07

40H12

EG374471

1249

F

Iron-sulfur cluster Isu1-like protein

gb|AAQ98966.1

Cryptococcus neoformans

8.00E-56

55E23a

EG374279

957

P

Mitochondrial ATPase alpha-subunit

gb|AAA33560.1

Neurospora crassa

6.00E-78

55E23b

EG374280

870

P

Mitochondrial ATPase alpha-subunit

gb|AAA33560.1

Neurospora crassa

1.00E-101

90M15

EG374409

1570

F

Mitochondrial carrier family protein

gb|EAK95613.1

Candida albicans

1.00E-46

30N15a

EG374419

1078

P

Succinate dehydrogenase flavoprotein subunit precursor

gb|AAW45324.1

Cryptococcus neoformans

1.00E-63

30N15b

EG374420

1143

P

Succinate dehydrogenase flavoprotein subunit precursor

gb|AAW45324.1

Cryptococcus neoformans

1.00E-136

10A2

EG374481

1114

F

V-type ATPase subunit G

gb|AAB41886.1|

Neurospora crassa

6.00E-15

7. Lipid metabolism

65D3

EG374370

1809

F

Diacylglycerol O-acyltransferase

gi|58268157

Cryptococcus neoformans

1.00E-84

65G21a

EG374424

1078

P

Fatty acid oxidoreductase

gb|AAW46114.1

Cryptococcus neoformans

2.00E-05

65G21b

EG374425

1149

P

Fatty acid oxidoreductase

gb|AAW46114.1

Cryptococcus neoformans

3.00E-32

58J11b

EG374309

732

P

Phosphatidyl synthase

gi|70999337

Aspergillus fumigatus

2.00E-20

8. Nucleotide metabolism

58C19a

EG374297

827

P

Uracil DNA N-glycosylase

gb|AAW41098.1

Cryptococcus neoformans

7.00E-16

58C19b

EG374298

857

P

Uracil DNA N-glycosylase

gb|AAW41098.1

Cryptococcus neoformans

1.00E-19

9. Protein modification

65B1

EG374366

1847

F

Carboxypeptidase

gi|19115337

Schizosaccharomyces pombe

7.00E-06

66B11a

EG374437

1145

P

Endopeptidase

gb|AAW41068.1

Cryptococcus neoformans

2.00E-69

66B11b

EG374438

1200

P

Endopeptidase

gb|AAW41068.1

Cryptococcus neoformans

1.00E-48

80N15

EG374397

1944

F

Translation elongation factor eEF-1 alpha chain

pir||S57200

Puccinia graminis

0.00E+00

10. Protein translational modification

55N13

EG374483

833

F

Ubiquitin-conjugating enzyme

ref|NP_594859.1

Schizosaccharomyces pombe

7.00E-21

11. Ribosomal protein complex

55B4

EG374472

770

F

16S small subunit ribosomal RNA

gi|52699765

Xanthoria elegans

2.00E-08

35O22

EG374462

938

F

18S ribosomal RNA

gi|21702995

Gymnosporangium libocedri

1.00E-154

60E22

EG374352

1117

F

18S ribosomal RNA

gi|34493860

Puccinia graminis f. sp.tritici

3.00E-142

65C12

EG374368

1136

F

18S ribosomal RNA

gi|34493860

Puccinia graminis f. sp.tritici

2.00E-66

90D5a

EG374432

1119

P

18S ribosomal RNA

gi|21724233

Puccinia striiformis f. sp.tritici

6.00E-102

90D5b

EG374431

1147

P

ITS1, ITS2 and 5.8S ribosomal RNA

gi|3668067

Tricholoma matsutake

9.00E-54

58E11b

EG374302

831

P

25S ribosomal RNA

gi|169606

Puccinia graminis f. sp. dactylis

1.00E-09

23H10b

EG374283

1921

F

28S ribosomal RNA

gi|37703614

Puccinia allii

1.00E-83

35M12a

EG374458

763

F

28S ribosomal RNA

gi|21724230

Puccinia graminis f. sp. tritici

2.00E-14

35N2

EG374460

917

F

28S ribosomal RNA

gi|46810582

Fuscoporia viticola

4.00E-06

35P13

EG374463

888

F

28S ribosomal RNA

gi|86160913

Melampsora epitea

2.00E-16

40A4

EG374464

951

F

28S ribosomal RNA

gi|58532805

Puccinia carthami

4.00E-05

55J11

EG374479

957

F

28S ribosomal RNA

gi|21724233

Puccinia striiformis f. sp. tritici

2.00E-26

35I10b

EG374502

422

P

28S ribosomal RNA

gi|21914221

Puccinia graminis

5.00E-77

35I22a

EG374505

716

P

28S ribosomal RNA

gi|21914221

Puccinia graminis

2.00E-70

35I22b

EG374504

878

P

ITS1, ITS2 and 5.8S ribosomal RNA

gi|21724233

Puccinia striiformis f. sp.tritici

5.00E-134

10G18

EG374323

1108

F

28S ribosomal RNA

gi|84452427

Cladosporium cladosporioides

1.00E-59

30C19

EG374333

1117

F

28S ribosomal RNA

gi|62005831

Puccinia ferruginosa

2.00E-13

30H3

EG374340

1052

F

28S ribosomal RNA

gi|21724233

Puccinia striiformis f. sp. tritici

3.00E-71

30I12

EG374341

1067

F

28S ribosomal RNA

gi|21724233

Puccinia striiformis f. sp. tritici

2.00E-39

30M20

EG374347

1008

F

28S ribosomal RNA

gi|21914221

Puccinia graminis

1.00E-93

60J23

EG374357

2112

F

calnexin

gb|AAS68033.1

Aspergillus fumigatus

1.00E-133

12. Sugar/glycolysis metabolism

30I15b

EG374418

617

P

Glucose-repressible protein

emb|CAC28672.1

Neurospora crassa

2.00E-14

90C20

EG374401

1130

F

Glucose-repressible protein

gi|70996962

Aspergillus fumigatus

7.00E-18

55J22b

EG374287

887

P

Glyoxal oxidase precursor

gb|AAW44259.1

Cryptococcus neoformans

2.00E-90

55J22a

EG374286

764

P

Glyoxal oxidase precursor

gb|AAW41343.1

Cryptococcus neoformans

3.00E-30

90H16

EG374405

1753

F

Phosphopyruvate hydratase

gi|1086120

Cladosporium herbarum

1.00E-139

30K8

EG374344

1547

F

Transaldolase

gb|AAW46393.1

Cryptococcus neoformans

3.00E-95

13. Transcription factor

58E6

EG374485

1310

F

TATA-box binding protein

gb|AAB57876.1

Emericella nidulans

7.00E-63

14. Transport metabolism

65M6

EG374378

1119

F

Cation transport-related protein

gb|AAW42114.1

Cryptococcus neoformans

3.00E-13

15. virulence/infection related protein

70I2

EG374433

1952

F

Cell wall glucanase

gi|70998053

Aspergillus fumigatus

2.00E-25

30M9

EG374345

1162

F

Differentiation-related/infection protein

gb|AAD38996.1

Uromyces appendiculatus

7.00E-11

80C7

EG374385

1180

F

Differentiation-related/infection protein

gb|AAD38996.1

Uromyces appendiculatus

1.00E-10

60E18

EG374351

2147

F

Pectin lyase

gb|AAA21817.1

Glomerella cingulata

2.00E-06


a F = full-length sequence and P = partial sequence.

Ling et al. BMC Genomics 2007 8:145   doi:10.1186/1471-2164-8-145

Open Data