Identification of p53-occupied genomic sites. A) Schematic of chromatin immunoprecipitation (anti-p53, FL-393) cloning strategy. See methods for more detail. B) Location of tags containing putative p53 binding sites relative to known genes within the rat genome. Binding sites scoring 90 or above out of 100 using the p53MH algorithm were included in this analysis. C) A hypothetical 100 kb gene map is used to show the relative location of 364 of experimentally determined p53 site-containing tags that achieved scores of ≥ 90 with the p53MH predictive binding algorithm. Data show that p53 site-containing tags were found in the upstream 5', downstream 3' and intergenic gene regions.
Brynczka et al. BMC Genomics 2007 8:139 doi:10.1186/1471-2164-8-139