Research article
Phase variation and microevolution at homopolymeric tracts in Bordetella pertussis
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* Corresponding author: Craig A Cummings craigc@stanford.edu
1 Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
2 Department of Medicine, Stanford University School of Medicine, Stanford, California 94305, USA
3 VA Palo Alto Health Care System, Palo Alto, California 94304, USA
4 2030 3rd. St., Unit #2, San Francisco, CA 94107, USA
5 Present address : University of California San Francisco, Biomedical Sciences Graduate Program, 513 Parnassus Ave., Room HSE-1285, San Francisco, CA, 94143, USA
BMC Genomics 2007, 8:122 doi:10.1186/1471-2164-8-122
Published: 17 May 2007Additional files
Additional file 1:
Supplementary Figure 1. Capillary electrophoresis traces of HPLC-purified bvgS LDR oligonucleotides.
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Additional file 2:
Supplementary Figure 2. Fidelity of Pfu and Taq DNA polymerases over multiple rounds of PCR/LDR.
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Additional file 3:
Supplementary Table 1. Characteristics of B. bronchiseptica HPTs
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Additional file 4:
Supplementary Table 2. Characteristics of B. pertussis HPTs
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Additional file 5:
Supplementary Table 3. Characteristics of B. parapertussis HPTs
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Additional file 6:
Supplementary Table 4. Characteristics of B. pertussis strains used in this study
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Additional file 7:
Supplementary Table 5. LDR and molecular weight standard oligonucleotides
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Additional file 8:
Supplementary Figure 3. BP0880 PCR/LDR.
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Additional file 9:
Supplementary Figure 4. sphB2 PCR/LDR.
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Additional file 10:
Supplementary Figure 5. bhuR PCR/LDR.
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Additional file 11:
Supplementary Figure 6. BP0059 PCR/LDR.
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Additional file 12:
Supplementary Figure 7. ptxA PCR/LDR.
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Additional file 13:
Supplementary Figure 8. fimX PCR/LDR.
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Additional file 14:
Supplementary Table 6. Lengths of HPTs in fim gene promoters and relative expression of fimX transcript.
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Additional file 15:
Supplementary Table 7. Lengths of HPT alleles detected by PCR/LDR for three hypervariable HPT loci.
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Additional file 16:
Supplementary Figure 9. BP3651 PCR/LDR.
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Additional file 17:
Supplementary Figure 10. tcfA PCR/LDR.
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Additional file 18:
Supplementary Figure 11. bapC PCR/LDR.
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Additional file 19:
Supplementary Figure 12. Bpe60 colonies screened for bapC HPT length
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Additional file 20:
Supplementary Table 8. PCR and RT-PCR oligonucleotide primers.
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