Table 1

Bacterical strains and plasmids

Strain or plasmid

Relevant characteristics

Source or reference


E. coli

S17-1/λpir

RK2 tra regulon, pir, host for pir-dependent plasmids

26

WG1

S17-1/λpir strain harboring suicide plasmid pWG3

This study

S. oneidensis

MR-1

Wild-type strain

2

DSP-10

Spontaneous rif ampin-resistant derivative of the MR-1, used as the reference strain in replace of MR-1 in this study

11

WG2

Transconjugant between E. coli WG1 and S. oneidensis DSP-10, containing a chromosomal integration of suicide plasmid pWG3

This study

WG3

In-frame S01377 gene deletion mutant of 1496-bp, derivative from WG2

This study

Plasmids

pCVD422

Suicide plasmid, bla oriT oriR6K sacB

27

pBSL142

pBluescript derivative, containing gentamicin -resistance gene

28

pDC3.O

Suicide plasmid generated by cloning the gentamicin-resistance gene from pBSL142 into the EcoRV site of pCV442

This study

pWG3

Suicide plasmid generated by cloning a 1.1 kb DNA fragment containing the mutated S01377 gene with 1496-bp deletion into the Sad site of pDS3.0.

This study


Gao et al. BMC Genomics 2006 7:76   doi:10.1186/1471-2164-7-76

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