Schematic presentation of generation of in-frame deletion mutant of SO1377 gene A. Generation of a deletion construct using asymmetric crossover PCR; B. Integration of the suicide plasmid during a single crossover event; C. Generation of in-frame deletion during the excision of the suicide plasmid. More detail about this construction process was described under "methods".
Gao et al. BMC Genomics 2006 7:76 doi:10.1186/1471-2164-7-76