Figure 3.

Multiple sequence alignment of TarB/TagB, TarF/TagF (protein) and their orthologs. Figure 3a. TarB/TagB orthologous ORFs in Mu50 and N315 (Mu50|gi|15923269 and N315|gi|1592316) are much shorter than in other strains (red arrow), which is suspected as an annotation error. So ORF finder program was rerun on this region and new_tarBs in Mu50 and N315 were identified. As shown in figure, new_tarB|Mu50 is a upstream extension of Mu50|gi|15923269. new_tarB|N315 is a upstream extension of N315|gi|1592316. See 1 for sequences of new_tarBs. Figure 3b. The size and homology clear shows that the analyzed S. aureus strains contain TarF like protein rather than TagF (blue arrow).